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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, Ca | WB, IP | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS1591 |
| Description | Anti-SR-alpha Antibody |
| Alternate Names |
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| Background Information | Signal recognition particle receptor subunit alpha (UniProt P08240; also known as Docking protein alpha, DP-alpha, SR-alpha, SRalpha) is encoded by the SRPR gene (Gene ID 6734) in human. Secretory proteins typically possess N-terminal hydrophobic signal sequences that direct them to the endoplasmic reticulum (ER) membrane, where they are translocated into the ER lumen for subsequent vesicular transport. Signal recognition particle (SRP) delivers target nascent secretory proteins, while they are still being synthesized, together with the associated ribosome to the ER protein translocon (Sec61 complex) via the interaction with SRP receptor (SR) on the ER membrane. SR is a heterodimer composed of a 70 kDa peripheral membrane protein (SR alpha subunit or SRα) and a 30 kDa singlel-trans membrane protein (SR beta subunit or SRβ). SRα contains an N-terminal SRX domain that mediates its association with SRβ and a C-terminal region which has high homology to the ng domain of SRP54, including a functional GTPase domain. SRβ also possesses a GTPase domain, which interacts with SRα SRX domain only when SRα is in the GTP bound state. SRα forms the major interaction interface with SRP. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-SR-alpha Antibody is validated for use in Western Blotting, Immunoprecipitation for the detection of SR-alpha. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: 3 µL from a represenative lot immunoprecipitated SRP receptor alpha subunit (SRα) from canine pancreas tissue rough microsome CHAP extract (Courtesy of Dr. Christopher V. Nicchitta, Duke University Medical Center, Durham, NC). Western Blotting Analysis: An 1:5,000 dilution of a representative lot detected SR-alpha in 15 µg HeLa cell lysate (Courtesy of Dr. Christopher V. Nicchitta, Duke University Medical Center, Durham, NC). Western Blotting Analysis: A representative lot detected SRP receptor alpha subunit (SRα) in the Brij35-sensitive (BrS), but not in the Brij35-resistant (BrR) fraction of HeLa ER membrfane proteins (Jagannathan, S., et Al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924). Western Blotting Analysis: A representative lot detected SRP receptor alpha subunit (SRα) in sodium cholate extract (CE) of ribosome-stripped canine pancreas rough microsome (RM) preparation (Migliaccio, g., et Al. (1992). J. Cell Biol. 117(1):15-25). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human SR-alpha. |
| Epitope | Internal (N-terminal half). |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Expected to react with both spliced isoforms of human SR-alpha reported by UniProt (P08240). |
| Species Reactivity |
|
| Species Reactivity Note | Human, Rat, Canine. Predicted to react with Mouse and Bovine based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~75 kDa observed. Target band size appears larger than the calculated molecular weights of 69.81 kDa (isoform 1) and 66.56 kDa (isoform 2). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in canine pancreas tissue rough microsome lysate. Western Blotting Analysis: An 1:500 dilution of this antibody detected SR-alpha in canine pancreas tissue rough microsome lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |