Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS1600 |
| Description | Anti-phospho-UVRAG (Ser498) |
| Alternate Names |
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| Background Information | UV radiation resistance-associated gene protein (UniProt: Q9P2Y5; also known as p63) is encoded by the UVRAG gene (Gene ID: 7405) in human. UVRAG is a key autophagic tumor suppressor that is mutated in several common human cancers. It contains four functional domains, a proline-rich domain, a lipid-binding C2 domain, a Beclin1-binding coiled-coil domain (CCD) and a C-terminal domain. It is a critical regulator of intracellular membrane trafficking, autophagy, and chromosomal stability. UVRAG is also involved in regulation of the COPI-dependent retrograde transport from Golgi and the endoplasmic reticulum by associating with the NRZ complex. Its function is dependent on its binding to phosphatidylinositol 3-phosphate (PtdIns3P). During autophagy UVRAG acts as regulatory subunit of the alternative PI3K complex II (PI3KC3-C2) that mediates formation of phosphatidylinositol 3-phosphate and is believed to be involved in maturation of autophagosomes and endocytosis. It is involved in maturation of autophagosomes and degradative endocytic trafficking independently of Beclin-1, but depends on its association with a class C Vps complex. UVRAG is also shown to enhance class C Vps complex association with a SNARE complex and promote fusogenic SNARE complex formation during late endocytic membrane fusion. UVRAG is also reported to promote DNA double-strand break repair by association with DNA-dependent protein kinase complex DNA-PK and activating it in non-homologous end joining. Disruption of UVRAG is shown to increase genetic instability and sensitivity of cells to irradiation. (Ref: Zhao, Z et al. (2012). Dev. Cell 22(5): 1001-1016). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect UV radiation resistance-associated gene protein using this rabbit polyclonal Anti-phospho-UVRAG (Ser498), Cat. No. ABS1600 that has been tested in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected phospho-UVRAG (Ser498) in TSC1 KO MEFs and treated with Torin1 (Kim, Y.M., et. al. (2015). Mol Cell. 57(2):207-18). Western Blotting Analysis: A representative lot detected phospho-UVRAG (Ser498) in HA-UVRAG+myc-Rheb2+DMSO. (Courtesy of Dr. Do-Hyung Kim at University of Minnesota). |
| Biological Information | |
|---|---|
| Immunogen | A linear peptide corresponding to 15 amino acids from the C-terminal half of human UVRAG surrounding phosphoserine 498. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects human UVRAG phosphorylated on Serine 498. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~98 kDa observed; 78.15 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cells transfected with HA-UVRAG+myc-Rheb2+DMSO (vehicle). Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-UVRAG (Ser498) in HEK293 cells transfected with HA-UVRAG+myc-Rheb2+DMSO (vehicle). |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |