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Millipore Detect Lip5 (Vacuolar Protein Sorting-Associated Protein Vta1 Homolog), Using This Rabbit Polyclonal Anti-Lip5, Cat. No. Abs1634, Validated For Use In Immunocytochemistry And Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Vacuolar protein sorting-associated protein VTA1 homolog
Dopamine-responsive gene 1 protein
DRG-1
LYST-interacting protein 5
LIP5
SKD1-binding protein 1
SBP1
Background Information
Vacuolar protein sorting-associated protein VTA1 homolog (UniProt: Q9NP79; also known as Dopamine-responsive gene 1 protein; DRG-1; LYST-interacting protein 5; LIP5; SKD1-binding protein 1; SBP1) is encoded by the VTA1 (also known as C6orf55; HSPC228; My012) gene (Gene ID 51534) in human. LIP5 is a 33.9 kDa protein involved in trafficking of the multi-vesicular body. It helps regulate the membrane fission and recycling activities of the ESCRT pathway. It performs its central regulatory function within the ESCRT pathway by binding ESCRT-III proteins through its N-terminal tandem microtubule-interacting and trafficking (MIT) domain and by binding VPS4 ATPases through its C-terminal VSL (Vta1/SBP1/LIP5) domain. These interactions allow LIP5 to coordinate ESCRT-III and VPS4 activities both during normal cellular homeostasis and also in response to interferon signaling during innate immune responses. The ESCRT-III and VPS4 ATPase complexes catalyze the membrane fission events associated with these processes. The LIP5 protein helps regulate their interactions by binding directly to a subset of ESCRT-III proteins and to VPS4.
Product Information
Format
Serum
Presentation
Rabbit polyclonal antibody serum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect LIP5 (Vacuolar protein sorting-associated protein VTA1 homolog), using this rabbit polyclonal Anti-LIP5, Cat. No. ABS1634, validated for use in Immunocytochemistry and Western Blotting.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected LIP5 in HeLa cells.
Western Blotting Analysis: A representative lot detected endogenous LIP5 co-immunoprecipiated with Myc-tagged VPS4A from HEK293T cells (Skalicky, J.J., et al. (2012). J Biol Chem. 287(52):43910-43926).
Biological Information
Immunogen
His-tagged full length human recombinant LIP5.
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
Detects LIP5 (Vacuolar protein sorting-associated protein VTA1 homolog) in HeLa cells
Species Reactivity
Human
Species Reactivity Note
Human.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_057569
Gene Symbol
VTA1
C6orf55
HSPC228
My012
Purification Method
Unpurified.
UniProt Number
Q9NP79
Molecular Weight
~38 kDa observed. 33.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antiserum detected LIP5 in HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
