Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS1634 |
| Description | Anti-LIP5 |
| Alternate Names |
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| Background Information | Vacuolar protein sorting-associated protein VTA1 homolog (UniProt: Q9NP79; also known as Dopamine-responsive gene 1 protein; DRG-1; LYST-interacting protein 5; LIP5; SKD1-binding protein 1; SBP1) is encoded by the VTA1 (also known as C6orf55; HSPC228; My012) gene (Gene ID 51534) in human. LIP5 is a 33.9 kDa protein involved in trafficking of the multi-vesicular body. It helps regulate the membrane fission and recycling activities of the ESCRT pathway. It performs its central regulatory function within the ESCRT pathway by binding ESCRT-III proteins through its N-terminal tandem microtubule-interacting and trafficking (MIT) domain and by binding VPS4 ATPases through its C-terminal VSL (Vta1/SBP1/LIP5) domain. These interactions allow LIP5 to coordinate ESCRT-III and VPS4 activities both during normal cellular homeostasis and also in response to interferon signaling during innate immune responses. The ESCRT-III and VPS4 ATPase complexes catalyze the membrane fission events associated with these processes. The LIP5 protein helps regulate their interactions by binding directly to a subset of ESCRT-III proteins and to VPS4. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal antibody serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect LIP5 (Vacuolar protein sorting-associated protein VTA1 homolog), using this rabbit polyclonal Anti-LIP5, Cat. No. ABS1634, validated for use in Immunocytochemistry and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected LIP5 in HeLa cells. Western Blotting Analysis: A representative lot detected endogenous LIP5 co-immunoprecipiated with Myc-tagged VPS4A from HEK293T cells (Skalicky, J.J., et al. (2012). J Biol Chem. 287(52):43910-43926). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged full length human recombinant LIP5. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Detects LIP5 (Vacuolar protein sorting-associated protein VTA1 homolog) in HeLa cells |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified. |
| UniProt Number |
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| Molecular Weight | ~38 kDa observed. 33.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: A 1:5,000 dilution of this antiserum detected LIP5 in HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |