Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Mk | WB, IF | Rb | Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS1645 |
| Description | Anti-Chromatin-Modifying Protein 6 |
| Alternate Names |
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| Background Information | Charged multivesicular body protein 6 (UniProt: Q96FZ7; also known as Chromatin-modifying protein 6, Vacuolar protein sorting-associated protein 20, Vps20, hVps20, CHMP6) is encoded by the CHMP6 (also known as VPS20) gene (Gene ID: 79643) in human. CHMP6 is a human orthologue of yeast Vps (vacuolar protein sorting) 20, a component of ESCRT (endosomal sorting complex required for transport)-III. Various CHMP6 orthologues in organisms ranging from yeast to humans contain the N-myristoylation consensus sequence at each N-terminus. CHMP6 is an ubiquitously expressed protein that has an acidic C-terminus and the basic N-terminus that render the protein in a closed, soluble and inactive conformation through an auto-inhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. CHMP6 is probable core component of the endosomal sorting required for transport complex III (ESCRT-III), which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Chromatin-Modifying Protein 6, Cat. No. ABS1645, is a rabbit polyconal antibody that is used to detect Chromatin-Modifying Protein 6 and has been tested for use in Immunofluorescence and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Chromatin-Modifying Protein 6 in Cos-7 cells (Langelier, C., et. al. (2006). J Virol. 80(19):9465-80). Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Chromatin-Modifying Protein 6 in HEK293 cell lysate (Langelier, C., et. al. (2006). J Virol. 80(19):9465-80). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged full length recombinant human Chromatin-modifying protein 6. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody detects chromatin-modifying protein 6 (CHMP6) in human and monkey cells. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human, Monkey. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~32 kDa observed; 23.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Chromatin-Modifying Protein 6 in 10 µg of HEK293 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |