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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, H | WB, IHC | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS1650 |
| Description | Anti-Gstp1 Antibody |
| Alternate Names |
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| Background Information | Glutathione S-transferase P (EC 2.5.1.18; UniProt P04906; also known as Chain 7, GST 7-7, GST class-pi) is encoded by the Gstp1 gene (Gene ID 24426) in rat species. Glutathione S-transferases constitute a family of metabolic isozymes that are involved in detoxification by catalyzing the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates. The GST family can be subdivided into the cytosolic, mitochondrial, and microsomal (a.k.a. MAPEG) proteins. Mammalian GSTs exist as either homodimers or heterodimers. The individual monomers were historically characterized by their quaternary structure and size, Yf (Mr 24.5 kDa), Yk (25 kDa), Ya1 (25.5 kDa), Ya2 (25.5 kDa), Yn (26.5 kDa), Yb1 (27 kDa), Yb2 (27 kDa) and Yc (28.5 kDa). The Y designation refers to the Y fraction (as apposed to the X and Z fractions) they were found in during purification by Sephadex G75 chromatography. Two GST superfamilies exist that comprise either cytosolic or membrane-bound proteins. Evidence suggests that cytosolic GST increases bodily resistance to carcinogens, products of oxidative stress, environmental pollutants and certain anti-cancer drugs. The cytosolic GSTs are further subdivided into eight classes. namely alpha (GSTA), mu (GSTM), pi (GSTP), sigma, theta (GSTT), kappa (GSTK), zeta (GSTZ), and omega (GSTO). GSTP exists as a homodimer composed of two Yf subunits. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal antibody in serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Gstp1 Antibody is an antibody against Gstp1 for use in Western Blotting, Immunohistochemistry. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected glutathione S-transferase P in rat liver and human stomach tissue homogenates (Cobbe,S.C., et Al. (2003). Eur. J. Gastroenterol. Hepatol. 15(1):41-47). Western Blotting Analysis: A representative lot detected upregulated glutathione S-transferase P (GSTP1) levels in liver homogenates from rats on diets supplemented with either one of six (EQ, CMRN, BHA, DEM, t-SO, or β-NF), but not with four other phytochemicals (OPZ, I3C, BITC, or PB) tested (Kelly, V.P., et Al. (2000). Cancer Res. 60(4):957–969). Immunohistochemistry Analysis: A representative lot detected much reduced glutathione S-transferase P (GSTP1) immunoreactivity in paraffin-embedded Barretts oesophagus tissues when compared to normal human oesophagus tissue sections (Cobbe,S.C., et Al. (2003). Eur. J. Gastroenterol. Hepatol. 15(1):41-47). Immunohistochemistry Analysis: A representative lot detected enhanced Glutathione S-transferase P immunoreactivity in the centrilobular part of liver from rats received dietary coumarin (CMRN) using acetone-fixed, paraffin-embedded tissue sections (Kelly, V.P., et Al. (2000). Cancer Res. 60(4):957–969). |
| Biological Information | |
|---|---|
| Immunogen | Purified rat liver glutathione S-transferase P. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Reacts with human and rat glutathione S-transferase P dimer (Yf/Yf) and monomer (Yf). |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~25 kDa observed (Yf monomer). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human lung tissue lysate. Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GSTP1 Yf monomer in 10 µg of human lung tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |