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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IH(P) | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS1653 |
| Description | Anti-GSTT1 Antibody |
| Alternate Names |
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| Background Information | Glutathione S-transferase theta-1 (EC 2.5.1.18; UniProt P30711; also known as Glutathione S-transferase T1-1, GST class-theta-1, GST T1-1) is encoded by the GSTT1 gene (Gene ID 2952) in human. Glutathione S-transferases constitute a family of metabolic isozymes that are involved in detoxification by catalyzing the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates. The GST family can be subdivided into the cytosolic, mitochondrial, and microsomal (a.k.a. MAPEG) proteins. Mammalian GSTs exist as either homodimers or heterodimers. The individual monomers were historically characterized by their quaternary structure and size, Yf (24.5 kDa), Yk (25 kDa), Ya1 (25.5 kDa), Ya2 (25.5 kDa), Yn (26.5 kDa), Yb1 (27 kDa), Yb2 (27 kDa) and Yc (28.5 kDa). The Y designation refers to the Y fraction (as apposed to the X and Z fractions) they were found in during purification by Sephadex G75 chromatography. Two GST superfamilies exist that comprise either cytosolic or membrane-bound proteins. Evidence suggests that cytosolic GST increases bodily resistance to carcinogens, products of oxidative stress, environmental pollutants and certain anti-cancer drugs. The cytosolic GSTs are further subdivided into eight classes. namely alpha (GSTA), mu (GSTM), pi (GSTP), sigma, theta (GSTT), kappa (GSTK), zeta (GSTZ), and omega (GSTO). GSTT1 exists as a homodimer, the subunit is referred to as subunit 5 in rat species, and T1-1 theta subunit in human. |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit polyclonal antibody in serum with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GSTT1 Antibody is an antibody against GSTT1 for use in Western Blotting, Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected recombinant human and murine GSTT1, as well as endogenous GSTT1 in human and mouse liver samples (Sherratt, P.J., et al. (2002). Toxicol. Appl. Pharmacol. 179(2):89-97), Western Blotting Analysis: A representative lot detected significantly upregulated GSTT1 levels in liver cytosolic preparations from rats on a selenium-deficient diet (McLeod, R., et al. (1997). Cancer Res. 57(19):4257-4266). Western Blotting Analysis: A representative lot detected recombinant human GSTT1, as well as endogenous human GSTT1 in multiple tissue samples from four deseased individuals (Sherratt, P.J., et al. (1997). Biochem. J. 326 (Pt 3):837-846). Immunohistochemistry Analysis: A representative lot detected differential GSTT1 immunoreactivity among liver cells in formalin-fixed, paraffin-embedded human and mouse liver tissue sections (Sherratt, P.J., et al. (2002). Toxicol. Appl. Pharmacol. 179(2):89-97). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged recombinant full-length human GSTT1. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Expected to react with both spliced isoforms reported by UniProt (P30711). Note that not all human samples are positive for GSTT1 expression. It has been estimated that approx. 18% of the Caucasian, and as much as 60% of the Chinese and Korean populations are GSTT1-null (Katoh, T., et al. (1996) Carcinogenesis. 17(9):1855-1859; Lear, J.T., et al. (1996) Carcinogenesis. 17(9):1891-1896;). |
| Species Reactivity |
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| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~27 kDa observed (monomer). 27.3 kDa (isoform 1) and 13.5 kDa (isoform 2) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in human liver tissue lysate. Western Blotting Analysis: A 1:5,000 dilution of this antibody detected glutathione S-transferase theta-1 (GSTT1) in 10 µg of human liver tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |