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Millipore Anti-Methyl-Glutamine, Cat. No. Abs2185, Is A Rabbit Polyclonal Antibody That Detects Glutamine Methylated Proteins. It Has Been Tested For Use In Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Glutamine methylation occurs on translation termination factors and ribosomal proteins. Protein methylation has been reported to affect various cellular processes including protein stability, protein-protein interaction, and protein localization. Methylation of histone is suggested as a platform to recruit and regulate other chromatin-related factors. Glutamine methylation is brought about by the action of protein glutamine methyltransferase HEMK2 that specifically requires a GQX3R motif for methylation activity. This antibody was tested for glutamine methylation in chromodomain helicase DNA-binding protein 5 (CHD5) that contains multiple PHD and chromo domains and is reported to function as a tumor suppressor.
Product Information
Format
Unpurified
Presentation
Rabbit polyclonal antiserum with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Methyl-Glutamine, Cat. No. ABS2185, is a rabbit polyclonal antibody that detects glutamine methylated proteins. It has been tested for use in Western Blotting.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected Methyl-Glutamine in CHD5 unmethylated & CHD5 methylated recombinant lysate (Coutesy of, Dr. Kusevic, Dr. Kuditipudi and Dr. Jeltsch at University Stuttgart, Germany).
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Methyl-Glutamine in in-vitro methylated CHD5, NUT, and eRF1 proteins (Kusevic, D., et. al. (2016). J Biol Chem. 291(12):6124-33).
Biological Information
Immunogen
KLH-conjugated Gly-Gln (Me)-Gly tripeptide.
Epitope
unknown
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects methylglutamine in proteins.
Isotype
IgG
Species Reactivity
All
Species Reactivity Note
Predicted to react with all species.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
N/A
Purification Method
Antiserum was depleted by running over unmethylated protein column.
UniProt Number
N/A
Molecular Weight
~51 kDa observed (for methylated CHD5). Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in methylated recombinant chromodomain helicase DNA-binding 5 (CHD5) protein, but not in unmethylated recombinant CHD5.
Western Blotting Analysis: A 1:3,000 dilution of this antibody detected Methyl-Glutamine in methylated recombinant chromodomain helicase DNA-binding 5 (CHD5) protein, but not in unmethylated recombinant CHD5.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
