| Description | |
|---|---|
| Catalogue Number | ABS2185-25UL |
| Description | Anti-Methyl-Glutamine |
| Background Information | Glutamine methylation occurs on translation termination factors and ribosomal proteins. Protein methylation has been reported to affect various cellular processes including protein stability, protein-protein interaction, and protein localization. Methylation of histone is suggested as a platform to recruit and regulate other chromatin-related factors. Glutamine methylation is brought about by the action of protein glutamine methyltransferase HEMK2 that specifically requires a GQX3R motif for methylation activity. This antibody was tested for glutamine methylation in chromodomain helicase DNA-binding protein 5 (CHD5) that contains multiple PHD and chromo domains and is reported to function as a tumor suppressor. |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Rabbit polyclonal antiserum with 0.05% sodium azide. |
| Applications | |
|---|---|
| Application | Anti-Methyl-Glutamine, Cat. No. ABS2185, is a rabbit polyclonal antibody that detects glutamine methylated proteins. It has been tested for use in Western Blotting. |
| Key Applications |
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| Application Notes | Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected Methyl-Glutamine in CHD5 unmethylated & CHD5 methylated recombinant lysate (Coutesy of, Dr. Kusevic, Dr. Kuditipudi and Dr. Jeltsch at University Stuttgart, Germany ). Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Methyl-Glutamine in in-vitro methylated CHD5, NUT, and eRF1 proteins (Kusevic, D., et. al. (2016). J Biol Chem. 291(12):6124-33). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated Gly-Gln (Me)-Gly tripeptide. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects methylglutamine in proteins. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Predicted to react with all species. |
| Antibody Type | Polyclonal Antibody |
| Purification Method | Antiserum was depleted by running over unmethylated protein column. |
| UniProt Number |
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| Molecular Weight | ~51 kDa observed (for methylated CHD5). Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in methylated recombinant chromodomain helicase DNA-binding 5 (CHD5) protein, but not in unmethylated recombinant CHD5. Western Blotting Analysis: A 1:3,000 dilution of this antibody detected Methyl-Glutamine in methylated recombinant chromodomain helicase DNA-binding 5 (CHD5) protein, but not in unmethylated recombinant CHD5. |
| Usage Statement |
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| Storage and Shipping Information | |
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| Storage Conditions | Stable for 1 year at -20°C from date of receipt. |
| Packaging Information | |
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| Material Size | 25 µL |