Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IH(P), IF | Rb | Unpurified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABS2192 |
| Description | Anti-mPTGES-1 |
| Alternate Names |
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| Background Information | Prostaglandin E synthase (UniProt: O14684; also known as EC:5.3.99.3, Microsomal glutathione S-transferase 1-like 1, MGST1-L1, Microsomal prostaglandin E synthase 1, MPGES-1, p53-induced gene 12 protein) is encoded by the PTGES gene (Gene ID: 9536) in human. mPGES -1 is a multi-pass membrane protein that is integral membrane, inducible and glutathione-dependent terminal synthase in the prostanoid biosynthesis pathway and acts downstream cyclooxygenases (COX) catalyzing the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Functionally, mPGES-1 is coupled to COX-2 and the induction of these two enzymes by pro-inflammatory cytokines, growth factors or endotoxin leads to a surge of PGE2 synthesis by inflammatory cells. It is strongly expressed with COX-2 in synovial fibroblasts and macrophages. In cases of osteoarthritis, mPGES-1 and COX-2 are strongly expressed in the chondrocytes and the synovial lining cells. mPGES-1 also play a role in the pathogenesis of ischemic stroke and many neurodegenerative diseases. In Alzheimer disease brains higher expression of both COX-2 and mPGES-1 is observed. Higher expression of mPGES has also been reported in several cancers and this higher expression has been correlated with vascular invasion and worse prognosis in colorectal cancer. A549 and DU145 xenografts lacking mPGES-1 display significantly reduced growth rates. (Ref.: Korotkova, M., and Jakobsson, PJ (2014). Basic Clin. Pharmacol. Toxicol. 114, 64-69). |
| Product Information | |
|---|---|
| Format | Unpurified |
| Presentation | Rabbit polyclonal antiserum without azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Prostaglandin E synthase using this rabbit polyclonal Anti-mPTGES-1, Cat. No. ABS2192 that has been tested in Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence, and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:5,000 dilution from a representative lot detected mPTGES-1 in human tonsil and human placenta tissue. Western Blotting Analysis: A representative lot detected mPTGES-1 in human chondrocytes (Tuure, L., et. al. (2015). Scand J Rheumatol. 44(1):74-9) and wild-type mixed glial cultures (Straccia, M., et. al. (2013). Glia. 61(10):1607-19). Immunofluorescence Analysis: A representative lot detected mPTGES-1 in Wild-type mixed glial cultures (Tuure, L., et. al. (2015). Scand J Rheumatol. 44(1):74-9). Western Blotting Analysis: A representative lot detected mPTGES-1 in PNE inhibitedmPGES-1proteinexpressioninIL-1 -stimulated SK-N-SH cells (Olajide, O.A., et. al. (2014). J Ethnopharmacol. 152(2):377-83). Immunohistochemistry Analysis: A representative lot detected mPTGES-1 in fresh frozen brain tissue (Tuure, L., et. al. (2015). Scand J Rheumatol. 44(1):74-9). |
| Biological Information | |
|---|---|
| Immunogen | His-tagged full-length recombinant human microsomal prostaglandin E synthase. |
| Epitope | unknown |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody specifically detects mPGES-1 in human. |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Mouse, Human. Predicted to react with Rat based on high sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~17 kDa observed; 17.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in recombinant microsomal Prostaglandin E synthase 1 protein. Western Blotting Analysis: A 1:10,000 dilution of this antibody detected mPTGES-1 in recombinant microsomal Prostaglandin E synthase 1 protein. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |