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Millipore This Anti-Glucagon Receptor Antibody Is Validated For Use In Western Blotting, Immunohistochemistry (Paraffin) For The Detection Of Glucagon Receptor. - Mill (Additional S&H or Hazmat Fees May Apply)
Glucagon receptor or GL-R (UniProt P47871) is encoded by the GCGR (also known as GGR) gene (Gene ID 2642) in human. Glucagon is a 29-amino acid peptide that stimulates glycogenolysis and gluconeogenesis in the liver to increase blood glucose. The receptor for glucagon is a class 2 (or class B) G protein-coupled receptor (GPCR) that signals through Gs to stimulate cAMP production. Mice lacking the glucagon receptor have mild hypoglycemia after fasting and exhibit hyperplasia of pancreatic alpha-cells. Because of its role in promoting hyperglycemia, the glucagon receptor presents a potential target for treatment of diabetes.
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Glucagon receptor Antibody is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Glucagon receptor.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Glucagon receptor in human pancreas.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Glucagon receptor.
Epitope
Fourth cytoplasmic domain
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Primate based on 100% sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NP_000151
Gene Symbol
GCGR
Purification Method
Affinity Purfied
UniProt Number
P47871
Molecular Weight
~59 kDa observed. Target band appears larger than the calculated molecular weight of 54.0 kDa (Preform) and 51.3 kDa (mature) due to glycosylation. Lower molecular weight degradation band(s) can also be seen (Iwanij, V., et al. (1990). J. Biol Chem. 265(34):21302-21308).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human liver tissue lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Glucagon receptor in 10 µg of human liver tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
