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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, IF | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT1340 |
| Description | Anti-Myosin-10/MYH10/Myosin IIB Antibody |
| Alternate Names |
|
| Background Information | Myosin-10 (UniProt P35580; also known as Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, NMMHC-B, NMMHC II-b, NMMHC-IIB, Non-muscle myosin heavy chain B, Non-muscle myosin heavy chain IIb) is encoded by the MYH10 (also known as NMMHCB) gene (Gene ID 4628) in human. Nonmuscle myosin II plays an important role in cell migration and invasion. The myosin II holoenzyme consists of two myosin heavy chains (MHCs), two essential light chains, and two regulatory light chains. Three different nonmuscle MHC II proteins exist in mammals, IIA (MYH9), IIB (MYH10), and IIC (MYH14), with both unique and overlapping roles. One of the initial steps in cancer cell metastasis is thought to be the process of epithelial–mesenchymal transition (EMT). Myosin IIB expression and myosin IIA heavy chain phosphorylation are upregulated upon TGF-β–induced EMT in mammary epithelial cells, while inhibition of myosin IIB expression in post-EMT mesenchymal cells reduces transmigration and invasion. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Myosin-10/MYH10/Myosin IIB Antibody is an antibody against Myosin-10/MYH10/Myosin IIB for use in Western Blotting, Immunocytochemistry, Immunofluorescence. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Myosin-10/MYH10/Myosin IIB in 10 µg of MCF-10A cell lysate. Western Blotting Analysis: A representative lot detected elevated MHC IIB expression in MDA-MB-468, MDA-MB-231, and MCF10A, while very low MHC IIB expression was detected in MCF7, T47D, BT-474, and SKBR3 (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996). Western Blotting Analysis: A representative lot detected MHC IIB levels in NMuMG murine mammary gland cells upon TGF-beta stimulation or shRNA-mediated hnRNP E1 knockdown. E1 overexpression obolished TGF-beta stimulated MHC IIB upregulation in NMuMG cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996). Immunocytochemistry Analysis: A representative lot detected MHC IIB immunoreactivity localized to the rear and perinuclear regions of migrating and transmigrating hnRNP E1-knockdown murine mammary gland E1-shRNA NMuMG cells by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed, 0.1% TritonX-100-permeablized cells (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996). Immunofluorescence Analysis: A representative lot detected MHC IIB immunoreactivity predominantly in the SAM-expressing myoepithelial layer, but not the cytokeratin 8-expressing luminal layer, in 4% paraformaldehyde-fixed, paraffin-embedded mouse mammary tissue sections by fluorescent immunohistochemstry (Beach, J.R., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(44):17991-17996). |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the C-terminal sequence of human myosin-10. |
| Epitope | C-terminus. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | Expected to react with all five spliced isoforms of human Myosin-10 reported by UniProt (P35580). |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
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| Molecular Weight | ~250 kDa observed. 229.0 kDa (isoform 1), 230.8 kDa (isoform 2), 231.4 kDa (isoform 3), 232.5 kDa (isoform 4), 230.0 kDa (isoform 5) calculated. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in A549 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myosin-10/MYH10/Myosin IIB in 10 µg of A549 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |