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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT1344 |
| Description | Anti-phospho-Keratin-20 Antibody (Ser13) |
| Alternate Names |
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| Background Information | Keratin, type I cytoskeletal 20 (UniProt P35900; also known as CK-20, Cytokeratin-20, K20, Keratin-20, Protein IT) is encoded by the KRT20 gene (Gene ID 54474) in human. Cytokeratin-20 (Keratin-20 or K20) belongs to a family of highly conserved epithelial-specific intermediate filament (IF) proteins that play an important role in the mechanical stability and integrity of epithelial cells and tissues. Each epithelium is characterized by its unique keratin expression pattern. All epithelial cells typically express at least one type-I and one type-II keratin, which form noncovalent heteropolymers. keratinocytes preferentially express K5/K14 or K1/K10 depending on their differentiation state in the epidermis, adult hepatocytes express K8/K18 exclusively, whereas intestinal epithelial cells express K8 as the major type II keratin with varying levels of the type I K18/K19/K20, depending on the differentiation state. Keratin 20 is a type I intermediate filament protein preferentially expressed in epithelia of the stomach, intestine, uterus, and bladder, as well as in skin Merkel cells. Because of its restricted tissue distribution, K20 is widely used as a marker to help assess the origin of metastatic tumors. K20 is phosphorylated on Ser13 during cellular apoptosis induction and tissue injury. PMA treatment is shown to induce cellular K20 Ser13 phosphorylation, implicating protein kinase C (PKC) as being one of the kinases that target the phosphorylation site. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rabbit polyclonal Anti-phospho-Keratin-20 (Ser13), Cat. No. ABT1344 is validated for use in Western Blotting for the detection of Keratin-20 Serine 13 phosphorylation. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human Keratin-20 target region sequence containing phosphorylated Ser13. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detected upregulated cellular Keratin-20 Ser13 phosphorylation upon phosphatase inhibitors treatment. Target site sequence is highly homologous, but not 100% conserved, among human, murine and rat species. |
| Species Reactivity |
|
| Species Reactivity Note | Human. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Modifications |
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| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~48 kDa observed. 48.49 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cells. Western Blotting Analysis: A 1:5,000 dilution of this antibody detected upregulated Keratin-20 Ser13 phosphorylation in 10 µg lysate from phosphatase inhibitors Calyculin A- and Okadaic acid-treated HeLa cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |