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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB, IHC, ICC, IP | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT1487 |
| Description | Anti-alpha-Actin Antibody, Smooth Muscle |
| Alternate Names |
|
| Background Information | Actin, aortic smooth muscle (UniProt P62736; also known as Alpha-actin-2, Cell growth-inhibiting gene 46 protein) is encoded by the ACTA2 (also known as AAT6, ACTSA, ACTVS, GIG46, MYMY5) gene (Gene ID 59) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-alpha-Actin Antibody, Smooth Muscle is an antibody against alpha-Actin for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 5.0 µg/mL from a representative lot immunostained smooth muscle alpha-Actin in NIH/3T3 fibroblasts, HUVECs, A431 and HeLa cells. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot immunostained smooth muscle cells in human uterus tissue sections. Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated smooth muscle alpha-Actin from 500 µg of C2C12 mouse myoblast lysate. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the N-terminual sequence of human smooth muscle alpha-actin. |
| Epitope | N-terminus. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody targets the N-terminal sequence specific to smooth muscle alpha-actin (ACTA2), but not skeletal muscle alpha-actin (ACTA1), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), or smooth muscle gamma-actin (ACTG2; ACTA3). |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat. Predicted to react with Chicken based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purfied |
| UniProt Number |
|
| Molecular Weight | ~42 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in C2C12 mouse myoblast lysate. Western Blotting Analysis: 0.25 µg/mL of this antibody detected smooth muscle alpha-Actin in 10 µg of C2C12 mouse myoblast lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |