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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | ICC | Rb | AlexaFluor®488 | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT1493-AF488 |
| Description | Anti-Cofilin-1 Antibody, Alexa Fluor® 488 Conjugate |
| Alternate Names |
|
| Background Information | Cofilin-1 (UniProt P23528; also known as 18 kDa phosphoprotein, Cofilin non-muscle isoform, p18) is encoded by the CFL1 (also known as CFL) gene (Gene ID 1072) in human. Cofilin-1 (non-muscle cofilin), cofilin-2 (muscle cofilin), and actin-depolymerizing factor (ADF) play an essential role in regulating actin filament dynamics and reorganization by stimulating the severance and depolymerization of actin filaments. The three cofilin family members share 70% to 81% sequence identity, exhibit similar biological functions, and display distinct but overlapping expression patterns. Cofilins and ADF bind actin monomers and filaments (G-actin and F-actin) and increase the number of actin monomers and filament fragments, facilitating filament turnover and treadmilling at key locations in migrating cells. Cofilins and ADF are inactivated by phosphorylation at Ser3 by LIM-kinases (LIMKs) and testicular protein kinases (TESKs) and are reactivated by dephosphorylation by the slingshot (SSH) family of protein phosphatases and chronophin. Cfl1-knockout results in mouse embryonic lethality at E11.5 12.5 due to aberrant neural tube closure and defective neural crest cell migration, while Adf-deficient mice are viable with normal brain appearance, but suffer from corneal defects that cause blindness in adult mice. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Presentation | Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rabbit polyclonal Anti-Cofilin-1 Antibody, Alexa Fluor® 488 Conjugate, Cat. No. ABT1493-AF488 is validated for use in Immunocytochemistry for the detection of cofilin-1. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected cofilin-1 in NIH/3T3 cells. The unconjugated antibody (Cat. No. ABT1493) is also available for immunocytochemistry, immunoprecipitation, and Western blotting applications. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to the N-termnal end sequence of human cofilin-1. |
| Epitope | N-terminus |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This Alexa Fluor® 488-conjugated polyclonal antibody targets cofilin-1 N-terminal end sequence conserved among bovine, human, mouse, porcine, and rat species. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Bovine, Porcine, Rat based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Affinity purified. |
| UniProt Number |
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| Molecular Weight | ~19 kDa observed. 18.39/18.37/184.3/18.39/18.40 kDa (bovine/human/mouse/porcine/rat) calculated (Met1 removed). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected cofilin-1 in HeLa cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |