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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | ABT15 |
| Description | Anti-ARHGEF6 |
| Alternate Names |
|
| Background Information | Rho guanine nucleotide exchange factor 6 (UniProt Q15052; also known as Alpha-Pix, COOL-2, PAK-interacting exchange factor alpha, Rac/Cdc42 guanine nucleotide exchange factor 6) is encoded by the ARHGEF6 (also known as COOL2, KIAA0006, MRX46, PIXA) gene (Gene ID 9459) in human. ARHGEF6 (alpha-Pix) belongs to a family of guanine nucleotide exchange factors (GEF) that activate the Rho family of small GTPases. ARHGEF6 acts as a Cdc42 and Rac1 GEF and forms a signling module with the adaptor GIT (GPCR kinase interactor), the GTPase effector PAK (p21 activated kinase), and the GTPase activator PIX (PAK-interacting exchange factor), facilitating the activation Rac and Cdc42 GTPases at specific subcellular locations. In contrast to the ubiquitious expression pattern of ARHGEF7 (beta-Pix), ARHGEF6 is only detected in highly motile cells, including immune cells, microvascular cells, and growing fetal neurons, where it plays an essential role in mediating cell spreading, directional migration, angiogenesis, and fetal neuron dendrite outgrowth. In addition, both ARHGEF6 and ARHGEF7 are shown to interact with the RING type E3 ubiquitin ligase c-Cbl. While ARHGEF6 is a substrate for c-Cbl-mediated proteasomal degradation, ARHGEF7 binds and antagonizes against c-Cbl-mediated EGFR proteasomal degradation. Elevated ARHGEF6 accumulation is found in human A172 and rat C6 glioma cells that do not express c-Cbl, resulting in enhanced cell migration and invasion. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rabbit polyclonal Anti-ARHGEF6 Antibody, Cat. No. ABT15 is validated for use in Immunocytochemistry and Western Blotting for the detection of Rho guanine nucleotide exchange factor 6. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:250 dilution from a represenative lot detected ARHGEF6 in A431, HeLa, and NIH/3T3 cells. Western Blotting Analysis: A 1:500 dilution from a represenative lot detected ARHGEF6 in 10 µg of human placenta tissue lysate as well as in 10 µg of A431, HeLa, HepG2, HUVEC, Jurkat, and PC3 cell lysates. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to an internal sequence C-terminal to the PH domain. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity | This polyclonal antibody targets an internal sequence C-terminal to the PH domain. |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. Predicted to react with Bovine, Rat, Rhesus Macaque based on 100% sequence homology. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Affinity purified. |
| UniProt Number |
|
| Molecular Weight | ~71 kDa observed. 87.50/70.79 kDa (human isoform 1/2), 87.05 kDa (mouse & rat) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HEK293 cell lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected ARHGEF6 in 10 µg of HEK293 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |