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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IF, IP, NEUT | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL1337-I |
| Replaces | CBL1337 |
| Description | Anti-PECAM-1 Antibody, clone 390 |
| Alternate Names |
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| Background Information | Platelet endothelial cell adhesion molecule (UniProt Q08481; also known as CD31, PECAM-1) is encoded by the Pecam1 (also known as Pecam, Pecam-1) gene (Gene ID 18613) in murine species. PECAM-1 (CD31) is a 130-kD cell adhesion molecule that is expressed at the junctions of all continuous endothelia, as well as most bone marrow-derived hematopoietic cells, including platelets, monocytes, granulocytes and some lymphocytes. The human PECAM1 and murine Pecam1 genes both produce multiple spliced isoforms with varying lengths in cytoplasmic domain, with the isoform Long being the major form expressed in human tissues and endothelial cells (ECs), and isoform delta 14-15 being the predominantly expressed murine form. PECAM-1 plays an import role in maintaining vascular integrity and function by mediating ECs interactions with pericytes and smooth muscle cells (SMCs). PECAM-1 is initially produced with a signal peptide (human a.a. 1-27, murine a.a. 1-17) sequence, the removal of which yields the mature protein with a large extracellular region (human a.a. 28-601, mouse a.a. 18-590) composed of six C2-type Ig-like domains, followed by a transmembrane seqment (human a.a. 602-620, mouse a.a. 591-609) and a cytoplasmic tail (human a.a. 621-738, mouse a.a. 610-727) that plays an essential role in PECAM-1-mediated cellular signaling. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat IgG2a in PBS without azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This rat monoclonal Anti-PECAM-1 Antibody, clone 390, Cat. No. CBL1337-I is validated for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Neutralization applications. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot detected the expression of wild-type, ∆12,15 and ∆14,15 murine PECAM-1 constructs on the surface of transfected mouse L-cells (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553). Immunofluorescence Analysis: A representative lot localized PECAM-1 immunoreactivity by fluorescent immunohistochemistry staining of murine embryo cryosections (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553). Immunoprecipitation Analysis: A representative lot immunoprecipitated the 120-130 kDa exogenously expressed PECAM-1 ∆12,15 construct from transfected murine L-cells (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553). Neutralizing Analysis: A representative lot inhibited the aggregation of murine L-cell transfectants expressing wild-type and ∆12,15, but not ∆14,15, murine PECAM-1 constructs (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553). Clone 390 is also available as FITC conjugate (Cat. No. CBL1337F) for direct immunofluorescent staining in flow cytometry, immunocytochemistry and immunohistochemistry applications. |
| Biological Information | |
|---|---|
| Immunogen | Mouse leukocyte cell line 32D. |
| Epitope | extracellular domain |
| Clone | 390 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone 390 immunoprecipitated the characteristic 120-130 kDa protein from L-cells transfected with muPECAM-1∆12,15 and selectively stained murine vessels in acetone-fixed tissue sections (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553). |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | 79.49/76.14/78.23/68.05 kDa (mature isoform 1/2/3/4), 81.26/77.92/80.00/69.82 kDa (isoform 1/2/3/4 pro-form) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in mouse splenocytes. Flow Cytometry Analysis: 2 µL of this antibody detected PECAM-1-positive monocytes among one million mouse splenocytes. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |