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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IP, ELISA, ICC, IHC, FUNC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL150-I |
| Description | Anti-CD3e Antibody, clone UCHT1 |
| Alternate Names |
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| Background Information | T-cell surface glycoprotein CD3 epsilon chain (UniProt P07766; also known as CD3-epsilon, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain) is encoded by the CD3E (also known as T3E) gene (Gene ID 916) in human. The T cell receptor or TCR is a plasma membrane protein complex responsible for recognizing antigens bound to major histocompatibility complex (MHC) on antigen presenting cells. In 95% of T cells, the TCR is composed of a disulfide-linked alpha (α) and beta (β) dimer, while among 5% of T cells the TCR is composed of a gamma (γ) and a delta (δ) dimer. T cells are activated when their surface TCRs engage with antigen/MHC complex on antigen presenting cells. Each TCR alpha and beta chain contains a variable (V) and a constant (C) extracellular immunoglobulin-like domain, followed by a transmembrane region and a short cytoplasmic tail. The alpha and beta chains have very short cytoplasmic tail and rely on the CD3 (cluster of differentiation 3) co-receptor complex for transducing signaling further intracellularly. The CD3 co-receptor complex is composed of a CD3γ chain, a CD3δ chain, and two CD3ε chains. The CD3 complex, TCR dimer, and a ζ-chain together comprise the functional TCR complex. The CD3 epsilon chain is initially produced with a signal peptide (a.a. 1-22), the removal of which yields the mature chain (a.a. 23-207) with an Ig-like (a.a. 32-112) domain-containing extracellular region (a.a. 23-126), a transmembrane segment (a.a. 127-152), and a cytoplasmic tail (a.a. 153-207), which contains the immunoreceptor tyrosine-based activation motif or ITAM (a.a. 178-205) that is essential for mediating ZAP-70 interaction and transducing TCR signaling. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in PBS without preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD3e Antibody, clone UCHT1 is an antibody against CD3e for use in Flow Cytometry, Immunoprecipitation, ELISA, Immunocytochemistry, Immunohistochemistry, Affects Function. |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: Representative lots immunoprecipitated CD3 epsilon-associated TCR complex (Schamel, W.W., et Al. (2005). J. Exp. Med. 202(4):493-503; Poggi, A., et Al. (1996). Int. Immunol. 8(12):1947-1953). ELISA Analysis: A representative lot was used as the capture antibody for the detection of epsilon-associated TCR complex proteins by sandwich ELISA (Poggi, A., et Al. (1996). Int. Immunol. 8(12):1947-1953). Immunocytochemistry Analysis: A representative lot detected the exogensously expressed human CD3 epsilon chain on the surface of murine T cells by flourescent immunocytochemistry (Clevers, H., et Al. (1988). Eur. J. Immunol. 18(5):705-710). Immunocytochemistry Analysis: A representative lot was employed to determined the plasma membrane and cytoplasmic CD3 immunoreactivity in T cell populations and T-ALL blast cells by flourescent immunocytochemistry (Campana, D., et Al. (1987). J. Immunol. 138(2):648-655). Immunohistochemistry Analysis: A representative lot detected CD3+ T cells (T3+) in the periglandular stromal aggregates in frozen dometrium sections from female donors undergone routine dilation and curettage procedures (Kamat, B.R., and Isaacson, P.g. (1987). Am. J. Pathol. 127(1):66-73). Affects Function: A representative lot induced surface IL-2R expression and proliferation of human peripheral blood T lymphocytes (Van Wauwe, J.P., et Al. (1984). J. Immunol. 133(1):129-132). Flow Cytometry Analysis: A representative lot was employed for fluorescence activated cell sorting (FACS) of CD3+ human lymphocytes (Beverley, P.C., and Callard, R.E. (1981). Science. 11(4):329-334). |
| Biological Information | |
|---|---|
| Immunogen | Human infant thymocytes and lymphocytes from a patient with Sézary disease. |
| Epitope | Extracellular domain. |
| Clone | UCHT1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone UCHT1 reacts with the extracellular domain of human CD3 epsilon chain (Clevers, H., et Al. (1988). Eur. J. Immunol. 18(5):705-710). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | 23.15 kDa (Proform) and 20.74 (mature form) calculated. ~27 kDa reported in UCHT1 immunoprecipitate (Poggi, A., et Al. (1996). Int. Immunol. 8(12):1947-1953). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in human PBMC. Flow Cytometry Analysis: 0.1 µg of this antibody detected CD3e in 1X10E6 human PBMC. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |