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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Ch, B | WB, IHC, ICC, IF, ELISA | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL171-I |
| Description | Anti-Actin, smooth muscle Antibody, clone ASM-1/1A4 |
| Alternate Names |
|
| Background Information | Actin, aortic smooth muscle (UniProt P62736; also known as Alpha-actin-2, Cell growth-inhibiting gene 46 protein) is encoded by the ACTA2 (also known as AAT6, ACTSA, ACTVS, GIG46, MYMY5) gene (Gene ID 59) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Actin, smooth muscle Antibody, clone ASM-1/1A4 is an antibody against Actin for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: A representative lot detected full-length and caspse-3-cleaved smooth muscle actin in uterus extracts from pregnant mice (Jeyasuria, P., et al. (2009). Biol. Reprod. 80(5):928-934). Western Blotting Analysis: A representative lot detected smooth muscle actin in PC-3M-LN4 and PC-3M-LN4-derived human prostate cancer cells (Zvieriev, V., et al. (2005). Biochem. Biophys. Commun. 337(2):498-504). Western Blotting Analysis: A representative lot detected developing stage-dependent vascular alpha-actin level in chicken heart of various somite stages (Ehler, E., et al. (2004). Dev. Dyn. 229(4):745-755). Western Blotting Analysis: A representative lot detected aortic actin, but not actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), or myocardium (alpha-myocardial) in various human, rat, bovine and rabbit tissue and cell lysates (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796). Immunohistochemistry Analysis: A representative lot immunostained smooth muscle actin-positive myofibroblasts in paraformaldehyde-fix rat skin wounds sections (Mirastschijski, U., et al. (2010). Wound Repair Regen. 18(2):223-234). Immunohistochemistry Analysis: A representative lot immunostained activated myofibroblasts in formalin-fixed, paraffin-embedded mouse submandibular salivary gland (Hall, B.E., et al. (2010). Lab. Invest. 90(4):543-555). Immunohistochemistry Analysis: A representative lot immunostained α-SMA-positive cells in paraffin-embedded rat healing patellar tendon sections (Fu, S.C., et al. (2008). J. Orthop. Res. 26(3):374-383). Immunocytochemistry Analysis: Representative lots immunostained the mesoderm layer of embryoid bodies formed in vitro from northern white rhinoceros iPSCs, as well as murine iPSCs and mESCs (Ben-Nun, I.F., et al. (2011). Nat. Methods. 8(10):829-831; Gerlach, J.C., et al. (2010). Cells Tissues Organs. 192(1):39-49; Shao, L., et al. (2009). Cell Res. 19(3):296-306). Immunohistochemistry Analysis: A representative lot immunostained a subsets of cultured rat aortic medial smooth muscle cells (SMCs) and dermal fibroblasts (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796). Immunohistochemistry Analysis: A representative lot immunostained cultured rat tendon cells (Fu, S.C., et al. (2008). J. Orthop. Res. 26(3):374-383). Immunofluorescence Analysis: A representative lot immunostained myofibrils using methanol-fixed, 9-somite stage chicken heart whole-mount preparations. The vascular alpha-actin staining started to disappear from a subset of the myofibrils when using 10-somite stage chicken heart preparations (Ehler, E., et al. (2004). Dev. Dyn. 229(4):745-755). Immunofluorescence Analysis: A representative lot immunostained stromal vessel SM cells in acetone-fixed benign (leiomyomas) and malignant (leiomyosarcomas & intravascular leiomyomatosis) smooth muscle (SM) neoplasms cryosections (Schürch, W., et al. (1987). Am. J. Pathol. 128(1):91-103). Immunofluorescence Analysis: A representative lot immunostained chicken gizzard and rat myocardium blood vessels, as well as smooth muscle cells (SMCs) in various acetone-fixed human and rat cryosections, whereas chicken gizzard parenchyrnal cells, rat cardiocytes, aorta endothelial cells and adventitial fibroblasts were negative (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796). ELISA Analysis: A representative lot detected aortic actin, but not actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), or myocardium (alpha-myocardial) by ELISA (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796). |
| Biological Information | |
|---|---|
| Immunogen | BSA-conjugated linear peptide corresponding to the N-terminal sequence of human smooth muscle actin. |
| Epitope | N-terminus. |
| Clone | ASM-1 (1A4) |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone ASM-1, also known as anti-αsm-1 and clone 1A4, reacted with aortic actin, but not actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial) by ELISA and Western blotting (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796). |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse, Rat, Chicken, Bovine. Predicted to react with Equine based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~45 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HUVEC lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected smooth muscle actin in 10 µg of HUVEC lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |