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Millipore Anti-Mcherry Antibody, Clone Ic51, Cat. No. Mab131873 Detects Mcherry Protein And Is Tested For Use In Immunocytochemistry And Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
mCherry, red fluorescent protein, is derived from DsRed, a red fluorescent protein from disc corals of Discosoma species. It is monomeric form that has been engineered with multiple cycles of mutation, directed modification and evolutionary selection. It exhibits excitation maximum at 587 nm and an emission maximum at 610 nm with a fluorescent quantum yield of 0.22. mCherry displays high photostability and resistant to photobleaching. It also displays improved brightness and extremely rapid maturation rate. Anti-mCherry, clone1C51 is an affinity purified antibody that recognizes mCherry in HEK293 cells transfected with pFin-EF1-mCherry. (Ref.: Schaner, NC et al. (2004). Nat. Biotechnol. 22(12):1567-72).
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG2a in buffer containing PBS with 0.03% sodium azide with 50% glycerol.
Quality Level
MQ100
Applications
Application
Anti-mCherry Antibody, clone IC51, Cat. No. MAB131873 detects mCherry protein and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
Immunocytochemistry
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected mCherry in Western Blotting applications (Krajcovic, M., et. al. (2013). Mol Biol Cell. 24(23):3736-45).
Western Blotting Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vs HEK293 untransfected cells (Courtesy of Dr Gerry Shaw).
Immunocytochemistry Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry (Courtesy of Dr Gerry Shaw).
Biological Information
Immunogen
His-tagged full length recombinant mCherry.
Clone
1C51
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 1C51 detects mCherry protein in multiple species.
Isotype
IgG2aκ
Species Reactivity
All
Species Reactivity Note
All
Antibody Type
Monoclonal Antibody
Gene Symbol
mCherry
Purification Method
Affinity Purified
UniProt Number
X5DSL3
Molecular Weight
~32 kDa Observed; 26.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HEK293 cells transfected with pFin-EF1-mCherry.
Western Blotting Analysis: 1 µg/mL of this antibody detected mCherry in 10 µg of lysate from HEK293 cells transfected with pFin-EF1-mCherry.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
