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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC | M | AlexaFluor®647 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1501-AF647 |
| Description | Anti-Actin Antibody, clone C4, Alexa Fluor® 647 conjugate |
| Alternate Names |
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| Background Information | Actins are ubiquitous eukaryotic proteins that serve as multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, transcriptional regulation, and vesicle trafficking. These functions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Beta- and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure and motility. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. The other four actin isoforms are typically found in specific adult muscle tissue types. alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. It has been shown that under calcium-bound conditions, beta-actin exhibits more dynamic behavior than gamma-actin with higher rates of polymerization and depolymerization. Also, beta- and gamma-actins can readily copolymerize, and the resulting filaments exhibit polymerization and depolymerization rates that vary depending on the ratio of beta- to gamma-actin (Lessard, JL., et al (1988). Cell Motility Cytoskeleton 10(3); 349-362). |
| Product Information | |
|---|---|
| Format | AlexaFluor®647 |
| Presentation | Purified mouse antibody conjugate in buffer containing PBS, 15 mg/mL BSA, and 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Actin Antibody, clone C4, Alexa Fluor® 647 conjugate is validated for use in Immunocytochemistry for the detection of Actin. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Actin in NIH/3T3 cells. The unconjugated version of this antibody (Cat. No. MAB1501) has been demonstrated to perform in WB, ELISA, ICC, IF, IHC, IH(P). |
| Biological Information | |
|---|---|
| Immunogen | Purified chicken gizzard actin (Lessard, 1988). |
| Epitope | Epitope is conserved in all known actins. |
| Clone | C4 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | MAB1501 is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts. Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988). |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Human. Predicted to react with Chicken based on 100% sequence homology. To date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | 40 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in HeLa cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Actin in HeLa cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |