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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC | M | AlexaFluor®647 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB19562-AF647 |
| Description | Anti-Laminin-5 Antibody (γ2 chain), clone D4B5, Alexa Fluor® 647 Conjugate |
| Alternate Names |
|
| Background Information | Laminin subunit gamma-2 (UniProt Q13753; also known as CSF 140 kDa subunit, Cell-scattering factor 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Ladsin 140 kDa subunit, Laminin B2t chain, Lamin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma) is encoded by the LAMC2 (also known as B2T, LAMB2T, LAMNB2) gene (Gene ID 3918) in human. Laminins are basement membrane, extracellular matrix glycoproteins composed of alpha, beta and gamma heterotrimeric chains. Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms. Laminin-5 (LN5), which consists of laminin alpha3, beta3 and gamma2 chains, is a highly conserved component of anchoring filaments underlying the hemidesmosome structure of epidermal keratinocytes. Mutations or deletion in the LN5 genes (LAMA3, LAMB3, and LAMC2) is associated with epidermolysis bullosa, a lethal skin blistering disease. LN5 may stabilize the epidermal/dermal junction of skin through interaction with integrins alpha3/beta1, alpha6/beta4 and type VII collagen and has a critical role in the anchorage and locomotion of a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. Comparing to fibronectin, collagen, and vitronectin, cells of epithelial origin will adhere to Laminin-5 faster and will spread to a larger extent. Laminin-5 attracted attention when it was detected at the leading edge of invasive cancer tissue in several types of human carcinomas (Niki, T., et al. (2002). Am. J. Pathol. 160(3):112911-41; Lohi, J. (2001). Int. J. Cancer. 94(6):763-767). |
| Product Information | |
|---|---|
| Format | AlexaFluor®647 |
| Presentation | Purified mouse antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Laminin-5 Antibody (γ2 chain), clone D4B5, Alexa Fluor® 647 Conjugate is an antibody against Laminin-5 for use in Immunocytochemistry. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Laminin-5 in NIH/3T3 cells. The unconjugated antibody (Cat. No. MAB19562) is shown to be suitable also for Western blotting, ELISA, and immunohistochemistry applications. |
| Biological Information | |
|---|---|
| Immunogen | Amino acid residues 382-608 of human laminin-5 gamma2 chain (III domain) expressed as a GST fusion protein. |
| Epitope | EGF-like 4 (second part) |
| Clone | D4B5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | This antibody recognizes the 150 (precursor form) and 105 kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| Molecular Weight | ~150 kDa calculated |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in A431 cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Laminin-5 in A431 cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |