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Tyrosine 3-monooxygenase (EC 1.14.16.2; UniProt P07101; also known as Dystonia 14, TH, Tyrosine 3-hydroxylase) is encoded by the TH (also known as DYT14, DYT5b, TYH) gene (Gene ID 7054) in human. Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of dopamine and norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. TH enzymatic activity requires ferrous ions as cofactor and is believed to be regulated by phosphorylation. Six isoforms of human TH exist as a result of alternative splicing (UniProt P07101-1 through P07101-6).
Product Information
Format
AlexaFluor®555
Presentation
Purified mouse monoclonal IgG1κ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality Level
MQ100
Applications
Key Applications
Immunocytochemistry
Application Notes
The unconjugated antibody (Cat. No. MAB318) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications.
Biological Information
Immunogen
Tyrosine Hydroxylase purified from PC12 cells.
Epitope
On the outside of the regulatory N-terminus of tyrosine hydroxylase.
Clone
LNC1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Recognizes an epitope on the outside of the regulatory N-terminus. The unconjugated antibody (Cat. No. MAB318) recognizes a protein of approximately 59-61 kDa by Western blotting. The unconjugated antibody does not react with the following by Western blotting: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT).
Isotype
IgG1κ
Species Reactivity
Zebrafish
Chicken
Frog
Human
Mouse
Rat
Vole
Monkey
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NM_000360.3
Gene Symbol
TH
TYH
DYT14
DYT5b
Purification Method
Protein A Purfied
UniProt Number
P07101
Molecular Weight
~59-63 kDa observed.
Product Usage Statements
Quality Assurance
Evaluated by Immunocytochemistry in rat cortical neurons.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Tyrosine Hydroxylase in rat cortical neurons.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
