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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Zebrafish, Ch, Fg, H, M, R, Vo, Mk | ICC | M | AlexaFluor®555 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB318-AF555 |
| Description | Anti-Tyrosine Hydroxylase Antibody, clone LNC1, Alexa Fluor® 555 Conjugate |
| Alternate Names |
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| Background Information | Tyrosine 3-monooxygenase (EC 1.14.16.2; UniProt P07101; also known as Dystonia 14, TH, Tyrosine 3-hydroxylase) is encoded by the TH (also known as DYT14, DYT5b, TYH) gene (Gene ID 7054) in human. Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of dopamine and norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. TH enzymatic activity requires ferrous ions as cofactor and is believed to be regulated by phosphorylation. Six isoforms of human TH exist as a result of alternative splicing (UniProt P07101-1 through P07101-6). |
| Product Information | |
|---|---|
| Format | AlexaFluor®555 |
| Presentation | Purified mouse monoclonal IgG1κ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Key Applications |
|
| Application Notes | The unconjugated antibody (Cat. No. MAB318) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications. |
| Biological Information | |
|---|---|
| Immunogen | Tyrosine Hydroxylase purified from PC12 cells. |
| Epitope | On the outside of the regulatory N-terminus of tyrosine hydroxylase. |
| Clone | LNC1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Recognizes an epitope on the outside of the regulatory N-terminus. The unconjugated antibody (Cat. No. MAB318) recognizes a protein of approximately 59-61 kDa by Western blotting. The unconjugated antibody does not react with the following by Western blotting: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| Molecular Weight | ~59-63 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in rat cortical neurons. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Tyrosine Hydroxylase in rat cortical neurons. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |