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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, B, H, M | IF, ICC | M | AlexaFluor®555 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB3420-AF555 |
| Description | Anti-Tau Antibody, clone PC1C6, Alexa Fluor® 555 Conjugate |
| Alternate Names |
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| Background Information | Microtubule-associated protein tau (UniProt P29172; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as TAU) gene (Gene ID 281296) in bovine species. Extracellular plaque deposits composed of amyloid-β and intracellular neurofibrillary tangles (NFTs) composed of truncated and hyperphosphorylated tau are two neuropathological hallmarks of Alzheimers disease (AD). Tau pathology (tauopathy) can cause toxicity when the brain is devoid of amyloid plaques, and tangle pathology correlates better with clinical dementia than amyloid pathology. Abnormal processing of tau by hyperphosphorylation and proteolytic truncation contribute to the toxicity of tau. Tau is known to be cleaved by various proteases, including caspases, calpains, cathepsins, thrombin, and puromycin-sensitive aminopeptidase, as well as the lysosomal cysteine proteinase asparagine endopeptidase (AEP). MAPK, GSK-3, and Cdk-5 are three known kinases that target various tau phosphorylation sites. Tau also undergoes other types of posttranslational modifications, including glycosylation, ubiquitination, glycation, polyamination, nitration, and lysine methylation, which are believed to be important for its non-pathological functions, including polymerization and stabilization of microtubules. |
| Product Information | |
|---|---|
| Format | AlexaFluor®555 |
| Presentation | Purified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Tau Antibody, clone PC1C6, Alexa Fluor® 555 Conjugate is validated for use in Immunofluorescence, Immunocytochemistry for the detection of Tau. |
| Key Applications |
|
| Application Notes | Immunofluorescence Analysis: A 1:300 dilution from a representative lot detected Tau in rat brain tissue. The unconjugated antibody (Cat. No. MAB3420) is shown to be suitable also for Western blotting and immunohistochemistry applications. |
| Biological Information | |
|---|---|
| Immunogen | Purified denatured bovine microtubule associated proteins. |
| Epitope | Internal (C-terminal half). |
| Clone | PC1C6 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone PC1C6 was originally designated as clone Tau-1. Clone PC1C6 (Tau-1) was initially thought to specifically target a form of tau present in axons by immunohistochemical staining of rat brain tissue (Binder, L.I. et Al. (1985). J. Cell Biol. 101(4):1371-1378), later studies revealed that its immunoreactivity and therefore tissue staining pattern depends on the state of tau phosphorylation. Specifically, clone Tau-1 displayed diminished immunoreactivity toward Tau when phosphorylated in its epitope region. Sample dephosphorylation by alkaline phosphatase treatment enhanced the immunoreactivity of this clone in Western blotting and immunohistochemistry applications (Papasozomenos, S.C., and Binder, L.I. (1987) Cell Motil. Cytoskeleton. 8(3):210-216; Szendrei, g.I., et Al. (1993). J. Neurosci. Res. 34(2):243-249); Billingsley, M.L., and Kincaid, R.L. (1997). Biochem. J. 323(Pt 3):577-591; Dotti, C.g., et Al. (1987). Neuroscience. 23(1):121-130). |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| Molecular Weight | ~79 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunofluorescence in mouse brain tissue. Immunofluorescence Analysis: A 1:150 dilution of this antibody detected Tau in mouse brain tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |