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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | ICC | M | AlexaFluor®488 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB374-AF488 |
| Description | Anti-GAPDH Antibody, clone 6C5, Alexa Fluor® 488 conjugate |
| Alternate Names |
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| Background Information | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Its binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP). As a house-keeping enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic role in metabolism, it is also involved in the initial stages of apoptosis or oxidative stress response where it is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH can also bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders, including the beta-amyloid precursor protein and the Huntingtin protein where reduced function of GAPDH is associated with Alzheimers and Huntingtons disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Presentation | Purified mouse conjugate antibody in buffer containing PBS 15 mg/mL BSA and 0.1% Sodium Azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-GAPDH Antibody, clone 6C5, Alexa Fluor® 488 conjugate is validated for use in Immunocytochemistry for the detection of GAPDH. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GAPDH in NIH/3T3 cells. The unconjugated version of this antibody (Cat. No. MAB374) has been demonstrated to perform in WB, ELISA, ICC, IHC, and IP. |
| Biological Information | |
|---|---|
| Immunogen | Glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle. |
| Clone | 6C5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from skeletal muscle. Antibody also recognizes cardiac GAPDH. GAPDH enzyme is detected in many non-muscle cells lines including HeLa, HCT-116 cells, U937 and THP-1 cells among others. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | Human and Mouse. Predicted to react with Porcine, Canine, Cat, Rat, Rabbit, and Fish based on 100% sequence homology. It has been reported that this antibody does not react with GAPDH from E. coli. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| Molecular Weight | ~38 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in A431 cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GAPDH in A431 cells. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |