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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, ICC, IF, IH(P), ChIP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB5434-C |
| Replaces | MAB5434 |
| Description | Anti-Oct-1 Antibody, clone YL15, Ascites Free |
| Alternate Names |
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| Background Information | POU domain, class 2, transcription factor 1 (UniProt P14859; also known as NF-A1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1, OTF-1) is encoded by the POU2F1 (also known as OCT1, OTF1) gene (Gene ID 5451) in human. Oct-1 belongs to the family of octamer transcription factors characterized by their binding to the ATTTGCAT octamer sequence via their POU domain (a.a. 280-354 of human Oct-1). Oct1 is widely expressed in adult tissues, however, Oct-1 knockdown has little impact on cell growth and viability in culture. Oct-1 controls multiple stem cell phenotypes in normal and tumor cells. Oct-1 promotes resistance to genotoxic and oxidative stresses in stem cells. Loss of Oct-1 antagonizes oncogenic transformation in vitro and tumorigenicity in vivo. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Oct-1 Antibody, clone YL15, Cat. No. MAB5434-C, is a mouse monoclonal antibody that detects octamer-binding protein 1 (Oct-1) and has been tested for use in immunocytochemistry, immunohistchemistry (Parffin), Immunoflouroesencece, Western blotting, and Chromatin Immunorecipitation. |
| Key Applications |
|
| Application Notes | Immunocytochemistry Analysis: 5.0 µg/mL from a representative lot detected Oct-1 cellular localization in human A431 and HeLa cells by fluorescent immunocytochemistry. Western Blotting Analysis: A representative lot detected Oct-1, but not Oct-2, Oct-4, or Oct-6 in various human cell lysates and nuclear extracts (Maddox, J., et al. (2012). PLoS Genet. 8(11):e1003048). Western Blotting Analysis: A representative lot detected Oct-1 in Burkitt lymphoma cells (BJAB and Namalwa), as well as a panel of six primary effusion lymphoma (PEL) cells (Di Bartolo, D.L., et al. (2009). J. Virol. 83(9):4308-4315). Immunofluorescence Analysis: A representative lot detected Oct-1 immunoreactivity in paraformaldehyde-fixed human and mouse frozen colon tissue sections by fluorescent immunohistochemistry (Maddox, J., et al. (2012). PLoS Genet. 8(11):e1003048). Immunohistochemistry Analysis: A representative lot detected Oct-1 immunoreactivity in formalin-fixed, paraffin-embedded human colon tissue sections (Maddox, J., et al. (2012). PLoS Genet. 8(11):e1003048). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Oct-1 occupancy at the ORF50 promoter, using stable Oct-2-inducible primary effusion lymphoma (PEL) cell line BC1/Tet-on/Oct-2. The association decreased upon induction of Oct-2 expression and enhanced upon ORF50 transfection (Di Bartolo, D.L., et al. (2009). J. Virol. 83(9):4308-4315). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Oct-1 occupancy at the U6 and U1 promoter regions in exponentially dividing HeLa cells (Zhao, X., et al. (2001). Mol. Cell. 7(3):539-549). Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of radiolabeled OCT1 consensus oligonucleotide-bound complex in the nuclear extracts from Burkitt lymphoma cells BJAB and Namalwa (Di Bartolo, D.L., et al. (2009). J. Virol. 83(9):4308-4315). Immunocytochemistry Analysis: A representative lot detected Oct-1 cellular localization in human diploid fibroblast MRC-5-derived HuS-L12 cells at various population-doubling levels (PDLs), as well as in the corresponding immortalized IML12–4 cells by fluorescent immunocytochemistry (Imai, S., et al. (1997). Mol. Biol. Cell. 8(12):2407-2419). Immunoprecipitation Analysis: A representative lot immunoprecipitated Oct-1 from HEK293 cell lysates (Lai, J.S., and Herr, W. (1992). Proc. Natl. Acad. Sci. U. S. A. 89(15):6958-6962). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant full-length human Oct-1. |
| Epitope | POU-domain linker region. |
| Clone | YL15 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone YL15 targets the POU-domain linker region (Herr, W., and Cleary, M.A. (1995). Genes Dev. 9(14):1679-1693; Lai, J.S., and Herr, W. (1992). Proc. Natl. Acad. Sci. U. S. A. 89(15):6958-6962) present in all six spliced isoforms of human Oct-1 (UniProt P14859). Clone YL15 does not react with Oct-2, Oct-4 or Oct-6 (Maddox, J., et al. (2012). PLoS Genet. 8(11):e1003048). |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~96 kDa observed. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected Oct-1 in 10 µg of HeLa cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |