Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | FC | AHm | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1031 |
| Description | Anti-TNFSF13 (APRIL) Antibody, clone A3D8 |
| Alternate Names |
|
| Background Information | Tumor necrosis factor ligand superfamily member 13 (UniProt: Q9D777; also known as A proliferation-inducing ligand; APRIL; CD256) is encoded by the Tnfsf13 (also known as APRIL) gene (Gene ID: 69583) in mouse. APRIL is a secreted cytokine of the tumor necrosis family and is present in T cells, B cells, dendritic cells, monocytes, and macrophages. April exists as a functional homotrimer, but can form functional heterotrimers with B cell activating factor (BAFF). APRIL stimulates T and B cell proliferation and it exerts T and B cell regulatory effects by binding to TNFRSF17 (BCMA) and TNFRSF13B (TACI) receptors. It is also involved in monocyte/macrophage-mediated immunological processes. It also plays a role in the regulation of tumor cell growth and can induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. APRIL can serve as as a potential biomarker and its serum levels are shown to be elevated for certain cancers and expression levels elevated in cancer tissues. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect TNFSF13 using this armenian hamster monoclonal Anti-TNFSF13 (APRIL) antibody, clone A3D8, Cat. No. MABC1031, validated for use in Flow Cytometry. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: 1 µg of this antibody from a representative lot detected TNFSF13 in one million RAW264.7 cells. |
| Biological Information | |
|---|---|
| Immunogen | A full length recombinant fusion protein of murine maltose binding protein (MBP) and soluble APRIL. |
| Clone | A3D8 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Armenian Hamster |
| Specificity | Clone A3D8 detects TNFSF13/APRIL in human peripheral blood mononuclear cells (PBMC). |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Human, Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G purified |
| UniProt Number |
|
| Molecular Weight | 26.89 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Flow Cytometry in human PBMC cells. Flow Cytometry Analysis: 1 µg of this antibody detected TNFSF13 in human PBMC cells. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |