Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1038 |
| Description | Anti-NQO2 Antibody, clone 4A4.1 |
| Alternate Names |
|
| Background Information | Ribosyldihydronicotinamide dehydrogenase [quinone] (EC 1.10.5.1; UniProt P16083; also known as NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, Quinone reductase 2, QR2) is encoded by the NQO2 (also known as NMOR2) gene (Gene ID 4835) in human. NQO1 (EC 1.6.5.2) and NQO2 (EC 1.10.5.1; formerly EC 1.10.99.2) constitute the quinone oxidoreductase family in human. NQO1 is involved in vitamin K metabolism and in reducing the cellular quinone concentration to prevent the build-up of cellular reactive oxygen species (ROS). Both enzymes contain a tightly bound FAD as the oxidant cofactor, but differ in their reductant cofactors utilization. While NQO1 can efficiently utilize NADH or NADPH, NQO2 exhibits only low activity in the presence of NADH or NADPH. Instead, non-physiological compounds, such as dihydronicotinamide riboside (NRH), N-methyldihydronicotinamide, and dihydrobenzylnicotamide, are reported to act as good reductant cofactors in NQD2-catalyzed reactions in vitro. NQO1 & NQO2 catalyzed the reduction of a wide variety of substrates, including quinones, nitro-compounds, and iron (III) ions. NQO1 is inhibited by the anticoagulant dicoumarol, while NQO2 is only weakly inhibited by dicoumarol and more strongly inhibited by resveratrol. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-NQO2 Antibody, clone 4A4.1 is an antibody against NQO2 for use in Western Blotting, Immunohistochemistry. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected NQO2 in 10 µg of Jurkat cell lysate. Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NQO2 in human kidney, liver, and cerebral cortex tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human NQO2 N-terminal fragment. |
| Epitope | Internal (N-terminal half). |
| Clone | 4A4.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 4A4.1 recognizes an internal epitope within the N-terminal half of human Quinone reductase 2. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | ~26 kDa observed. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected NQO2 in 10 µg of HeLa cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |