Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1145 |
| Description | Anti-FAP Antibody, clone 73.3 |
| Alternate Names |
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| Background Information | Fibroblast activation protein (UniProt: ; also known as Propyl endopeptidase FAP, Dipeptidyl peptidase FAP, Serine integral membrane protease, SIMP) is encoded by the Fap gene (Gene ID: 14089) in murine species. FAP is a homodimeric type II transmembrane protein that physically attaches to cells and bulk of the protein is exposed to extracellular space. It is a gelatinase of the serine protease family that is selectively expressed by tumor associated fibroblasts and pericytes in a majority of human epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. It is not expressed in most normal tissues, but is expressed during embryonic development. It is present as a homodimer and homodimerization is required for activity of both plasma membrane and soluble forms. It can also form heterodimer with DPP4. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences. Its gelatinase activity is inhibited by several serine-protease inhibitors, such as PMSF, AEBSF, and diisopropyl fluorophosphate (DFP). FAP is involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. It is also involved in the cell invasiveness towards the ECM in malignant melanoma cancers. (Ref.: Kelly, T et al. (2012). Int. Rev. Cell Mol. Biol. 297:83-116; Santos, AM et al. (2009). J. Clin. Invest. 119(12):3613-3625). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect fibroblast activation protein using this mouse monoclonal Anti-FAP Antibody, clone 73.3, Cat. No. MABC1145. It has been tested in Flow Cytometry. |
| Key Applications |
|
| Application Notes | Flow Cytometry Analysis: A representative lot detected FAP in Flow Cytometry applications (Wang, L.C., et. al. (2014). Cancer Immunol Res. 2(2):154-66). Flow Cytometry Analysis: 1 µg/mL from a representative lot detected FAP in 3T3/FAP overexpressed cells, but not in 3T3 WT cells. (Courtesy of Leslie Hopper, University of Pennsylvania, USA). |
| Biological Information | |
|---|---|
| Immunogen | FAP-expressing 3T3 cells. |
| Clone | 73.3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 73.3 specifically detects fibroblast activation protein (FAP) in 3T3 cells overexpressing FAP. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified |
| UniProt Number |
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| Molecular Weight | 87.94 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Isotype testing: Identity Confirmation by Isotyping Test. Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 . |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |