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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC1166 |
| Description | Anti-iASPP Antibody, clone LX128.5 |
| Alternate Names |
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| Background Information | RelA-associated inhibitor (UniProt Q8WUF5; also known as Inhibitor of apoptosis-stimulating protein of p53, Inhibitor of ASPP protein, NF-kB-interacting protein 1, PPP1R13B-like protein, Protein iASPP) is encoded by the PPP1R13L (also known as IASPP, NKIP1, PPP1R13BL, RAI) gene (Gene ID 10848) in human. Inhibitor of apoptosis-stimulating protein of p53 (iASPP) belongs to the ASPP family of proteins (ASPP1, ASPP2, and iASPP) characterized by their ankyrin repeats (a.a. 659-691 & 692-724 in iASPP), SH3 domain (a.a. 758-820 in iASPP), and proline-rich (a.a. 54-602 in iASPP) sequences. iASPP is expressed at intercalated discs in post-mitotic cardiomyocytes, where it interacts with desmoplakin and desmin in cardiomyocytes to help maintain the integrity of desmosomes and intermediate filament networks. Desmosomes are anchoring junctions found in cells that endure physical stress such as cardiac myocytes or skin keratinocytes. Mutations of genes coding desmosome components, including IASPP/PPP1R13L, are found in human patients suffering from cardiomyopathy and in animal models of cardiomyopathy. iASPP deficiency caused by spontaneous mutation has been reported to be linked to a lethal autosomal recessive cardiomyopathy in Poll Hereford calves and Wa3 mice. iASSP-deficient mice with exon 8 deletion display features of arrhythmogenic right ventricular cardiomyopathy (ARVC) and die of sudden cardiac death. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-iASPP Antibody, clone LX128.5 is an antibody against iASPP for use in Immunohistochemistry (Paraffin). |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A representative lot immunostained intercalated discs in myocardial sections from adult wild-type, but not iASPP-deficient (iASPPΔ8/Δ8) mice (Notari, M., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(9):E973-E981). Note: iASPP is expressed at intercalated discs. To prepare heart tissue sections for iASPP staining, it is important to cut the tissues at an angle parallel to the plane of the intercalated discs. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic linear peptide corresponding to a sequence from the N-terminal region of human iASPP. |
| Epitope | N-terminal region. |
| Clone | LX128.5 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone LX128.5 immunostained intercalated discs in myocardial sections from wild-type, but not iASPP-deficient (iASPPΔ8/Δ8) adult mice (Notari, M., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(9):E973-E981). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Species Reactivity Note | Mouse. Predicted to react with Human based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | 89.09 kDa calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunohistochemistry in mouse heart tissue. Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected iASPP in mouse heart tissue. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |