The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Detect Arhgap36 Using This Mouse Monoclonal Anti-Arhgap36, Clone 12d10.1 Antibody, Cat. No. Mabc1216, Validated For Use In Immunohistochemistry (Paraffin) And Western Blotting. - Mill (Additional S&H or Hazmat Fees May Apply)
Rho GTPase-activating protein 36 (UniProt Q6ZRI8) is encoded by the ARHGAP36 gene (Gene ID 158763) in human. ARHGAP36 is a Rho GAP family member that acts as a potent hedgehog (Hh) pathway positive regulator. ARHGAP36 is reported to functionally associate with Hh pathway negative regulator Sufu (suppressor of fused) and ARHGAP36 overexpression results in reduced Gli3R levels, Gli2 ciliary accumulation, and Gli1 expression, in a Smo-independent manner. ARHGAP36 is produced with a signal peptide sequence (a.a. 1-40), which is removed posttranslationally to yield the mature protein with a Rho-GAP domain (a.a. 226-426). Unlike other Rho GAP domain-containing proteins, ARHGAP36 lacks the arginine finger motif that stimulates GTPase hydrolysis and GAP domain mutants can retain Gli-activating activity. ARHGAP36 isoforms are up-regulated in a subset of medulloblastomas, where they mediate noncanonical Gli activation by inhibiting Sufu.
Product Information
Format
Purified
Presentation
Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Arhgap36 using this mouse monoclonal Anti-ARHGAP36, clone 12D10.1 Antibody, Cat. No. MABC1216, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ARHGAP36 in human prostate cancer, adrenal gland and pancreas tissue sections.
Biological Information
Immunogen
GST-tagged recombinant human ARHGAP36 C-terminal fragment.
Clone
12D10.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 12D10.1 epitope lies within a C-terminal region present in all five human ARHGAP36 spliced isoforms reported by UniProt (Q6ZRI8).
Evaluated by Western Blotting in Arhgap36 recombinant protein.
Western Blotting Analysis: A 1:500 dilution of this antibody detected 50 ng of GST-tagged recombinant human ARHGAP36 C-terminal fragment.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
