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Millipore This Anti-Somatoliberin Antibody, Clone 1b4.2 Is Validated For Use In Western Blotting And Immunohistochemistry (Paraffin) For The Detection Of Ghrh . - Mill (Additional S&H or Hazmat Fees May Apply)
The human GHRH (also known as GHRF) gene (Gene ID 2691) encodes the 108 aa Somatoliberin preproprotein (UniProt P01286; also known as GHRH, GRF, Growth hormone-releasing factor, Growth hormone-releasing hormone, INN: Sermorelin, Sermorelin, Somatocrinin, Somatorelin). Post-translatioinal removal of the signal peptide (aa 1-20) and the two propeptide sequences (aa 21-31 and aa 76-108) releases the 44 aa mature Somatoliberin (aa 32-75) that functions as a ligand to stimulate growth hormone (GH) release from the pituitary. Variant GHRH receptors are found in several types of tumors. Antagonists against such receptors are reported to inhibit tumors growth. Defects in GHRH gene cause dwarfism and hypersecretion of somatoliberin is linked to gigantism.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Somatoliberin Antibody, clone 1B4.2 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of GHRH .
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Somatoliberin/GHRH in human cerebral cortex tissue.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to mature human somatoliberin sequence.
Epitope
Mature somatoliberin
Clone
1B4.2
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 1B4.2 is expected to detect prepro-, pro-, and mature somatoliberin.
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001171660
Gene Symbol
GHRH
GHRF
Purification Method
Protein G Purified
UniProt Number
P01286
Molecular Weight
~12 kDa observed. Preprosomatoliberin (12.4 kDa calculated); Prosomatoliberin (10.9 kDa calculated); Mature somatoliberin (10.4 kDa calculated). Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human lung tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Somatoliberin/GHRH in 10 µg of human lung tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
