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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC582 |
| Description | Anti-hIAP-1/BIRC3 Antibody, clone 11D12.1 |
| Alternate Names |
|
| Background Information | Baculoviral IAP repeat-containing protein 3 (UniProt Q13489; also known as API2, Apoptosis inhibitor 2, Baculoviral IAP repeat-containing 3, c-IAP2, hIAP-1, hIAP1, IAP homolog C, IAP-1, Inhibitor of apoptosis protein 1, Mammalian IAP homolog C, RING finger protein 49, TNFR2-TRAF-signaling complex protein 1) is encoded by the BIRC3 (also known as API2, AIP1, IAP1, HAIP1, HIAP1, MALT2, MIHC, RNF49) gene (Gene ID 330) in human. hIAP-1 (c-IAP2) belongs to the inhibitor of apoptosis (IAP) family of proteins characterized by the presence of at least one baculoviral IAP repeat (BIR) domain. hIAP-1 (c-IAP2 contains three BIR (BIR1/2/3) domains, followed by an UBA (ubiquitin-associated), a CARD (caspase activation and recruitment domain), and a RING (really interesting new gene) domain. The BIR domains mediate hIAP-1 interaction with various binding partners, such as caspases, other IAP family members, and receptor-associated complexes. The RING domain and its associated E3 ligase mediates not only the ubiqutination of substrate proteins, such as caspase-3, caspase-7, SMAC/DIABLO, but also self-ubiquitylation. The UBA domain enables hIAP-1 to engage protein complexes containing poly-ubiquitylated protein(s). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect BIRC3 using this mouse monoclonal Anti-hIAP-1/BIRC3 Antibody, clone 11D12.1, Cat. No. MABC582, validated for use in Western Blotting. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.0 µg/mL from a representative lot detected hIAP-1/BIRC3 in 10 µg of TF-1, Jurkat, and Molt-4 cell lysate. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to the BIR 1/2 of human hIAP-1/BIRC3. |
| Clone | 11D12.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein g Purified |
| UniProt Number |
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| Molecular Weight | ~60 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in K562 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected hIAP-1/BIRC3 in 10 µg of K562 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |