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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC609 |
| Description | Anti-Thymidylate Kinase/TMPK Antibody, clone 1A5.1 |
| Alternate Names |
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| Background Information | Thymidylate kinase (EC 2.7.4.9; UniProt P23919; also known as dTMP kinase) is encoded by the DTYMK (also known as CDC8, PP3731, TMPK, TYMK) gene (Gene ID 1841) in human. Thymidylate kinase (TMPK) is an ubiquitously expressed enzyme of the dTTP synthesis pathway involved in DNA synthesis. TMPK catalyzes the phosphorylation of thymidine 5-monophosphate (dTMP) in the presence of ATP and Mg(2+) to form thymidine 5-diphosphate (dTDP), which is then further phosphorylated by nucleoside-diphosphate kinase (NDK) to thymidine 5-triphosphate (dTTP). Aside from its normal physiological role, TMPK activity is essential for the activation of nucleoside analogs-based prodrugs, such as the clinically used anti-HIV prodrugs AZT and d4T, which need to be converted to the triphosphate forms for their pharmacological activities. Inhibition of TMPK activity is reported to augment the potency of anticancer agent doxorubicin against human colon cancer cells regardless of their p53 status. In addition, lung cancers with LKB1-mutations are reported to show deficits in nucleotide metabolism and exhibit hypersensitivity to DTYMK inhibition. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-TMPK Antibody, clone 1A5.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Thymidylate Kinase . |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Thymidylate Kinase/TMPK in 10 µg of MCF-7 cell lysate. Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Thymidylate Kinase/TMPK in human breast cancer and human ovarian cancer tissue. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human Thymidylate Kinase/TMPK. |
| Clone | 1A5.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~24 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in Jurkat cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Thymidylate Kinase/TMPK in 10 µg of Jurkat cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |