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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P), WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABC785 |
| Replaces | MABC672 |
| Description | Anti-SOX9 Antibody, clone 4B7.1 |
| Alternate Names |
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| Background Information | Transcription factor SOX-9 (UniProt P48436; also known as Sex-determining region Y-box 9 protein, SRY-related HMG-box gene 9) is encoded by the SOX9 (also known as CMD1, CMPD1, SRA1) gene (Gene ID 6662) in human. Sox family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences encoding for the HMG-box domains that mediate sequ+D11ence-specific DNA binding. SOX genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at at least 40 genomic loci and at least 30 Sox genes have been identified. SOX-9 is a key regulator of developmental processes, including male sex determination, chondrogenesis, neurogenesis, and neural crest development. SOX-9 up-regulation is reported in cancers of various origins, including breast, colon, prostate, and lung, where its expression correlates with malignancy and patient survival. Cellular SOX9 mRNA is shown to be targeted and negatively regulated+D11 by miR-32, and the cellular miR-32 level is reported to be significantly lower in non-small cell lung cancer (NSCLC) compared with noncancerous lung tissues. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect SOX9 using this mouse monoclonal Anti-SOX9, clone 4B7.1 Antibody, Cat. No. MABC785, validated for use in Immunohistochemistry (Paraffin) and Western Blotting. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected SOX9 in human liver cancer and small intestine tissue sections. |
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to human SOX9 C-terminal end sequence. |
| Clone | 4B7.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 4B7.1 epitope lies within the highly conserved C-terminal end region of SOX9. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Chicken, Mouse, Non-Human Primate, Porcine, Rat based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G purified. |
| UniProt Number |
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| Molecular Weight | ~69 kDa observed. 54.85/56.14/56.08/56.12/56.12 kDa (chicken/human/mouse/porcine/rat) calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SOX9 in 10 µg of HepG2 cell lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |