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Millipore Anti-N6-Methyladenosine Antibody (M6a), Clone 17-3-4-1 Is An Antibody Against N6-Methyladenosine For Use In DOT Blot And Rna Binding Protein Immunoprecipitation. - Mill (Additional S&H or Hazmat Fees May Apply)
N6-Methyladenosine (m6A ) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. Fat mass and obesity-associated (FTO) and ALKBH5 are two identified demethylases that catalyze oxidative demthylation of m6A. Several functions of m6A modification have been proposed, including mRNA decay, splicing regulation, RNA nuclear export and maturation. (PMID 25171402). Cat. No. MABE1006, Anti-N6-methyladenosine (m6A), clone 17-3-4-1, is a mouse monoclonal that specifically detects RNA molecules with m6A modication.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-N6-methyladenosine Antibody (m6A), clone 17-3-4-1 is an antibody against N6-methyladenosine for use in DOT Blot and RNA binding protein immunoprecipitation.
Key Applications
DOT Blot
RNA Binding Protein Immunoprecipitation (RIP)
Application Notes
(RNA) Immunoprecipitation Analysis: A representative lot immunoprecipitated N6-methyladenosine in IME2 transcripts (Bodi, Z., et Al. (2010). Nucleic Acids Research. 38(16):5327-5335). RNA DOT Blot Analysis: A representative lot detected N6-methyladenosine in m6A (Bodi, Z., et Al. (2010). Nucleic Acids Research. 38(16):5327-5335).
Biological Information
Immunogen
Hapten N6-methyladenosine-5-mono-phosphate conjugated to BSA of all N6-methyladenosine (m6A).
Epitope
N6-methyladenosine
Clone
17-3-4-1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG1λ
Species Reactivity
All
Antibody Type
Monoclonal Antibody
Purification Method
Protein g Purified
Product Usage Statements
Quality Assurance
Evaluated by RNA DOT Blot in oligomers containing N6-methyladenosine.
RNA DOT Blot Analysis: 1.0-2.0 μg/mL of this antibody detected N6-methyladenosine in oligomers containing N6-methyladenosine.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
