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Millipore Anti-Poly-ADP-Ribose Binding Reagent Is A Reagent That Selectively Binds To ADP Ribose For Use In Western Blotting, Immunocytochemistry And DOT Blot. - Mill (Additional S&H or Hazmat Fees May Apply)
Cat. No. MABE1031, Anti-poly-ADP-ribose binding reagent, is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta™(DE3)pLysS strain of E. coli (Cat. No. 70956). Anti-poly-ADP-ribose binding reagent is useful for the affinity detection of oligo- and poly-ADP-ribosylated (PARylated) proteins on membranes or on fixed cells in a manner similar to antibody-based Western blot, DOT blot, and immunocytochemistry applications. The rabbit Fc tag allows visualization of the binding/labeling with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-poly-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.
Product Information
Format
Purified
Presentation
Purified from E. coli by Ni-NTA agarose. Supplied in buffer containing 10 mm Tris pH 7.5, 0.2 M NaCl, 10% Glycerol, 10 mm Imidazole, 1 mm PMSF, 1 mm β-Mercaptoethanol, 10% glycerol without preservatives.
Quality Level
MQ100
Applications
Application
Anti-poly-ADP-ribose binding reagent is a reagent that selectively binds to ADP ribose for use in Western Blotting, Immunocytochemistry and DOT Blot.
Key Applications
Western Blotting
Immunocytochemistry
DOT Blot
Application Notes
DOT Blot Specificity Analysis: This reagent detected oligo(ADPR) and poly(ADPR) on ADP-ribosylated PARP1 recombinant protein (Lee Kraus, University of Texas Southwestern Medical Center). Immunocytochemistry Analysis: A representative lot detected oligo(ADPR) and poly(ADPR)/PAR in 3T3-L1 cells (Lee Kraus, University of Texas Southwestern Medical Center).
Biological Information
Concentration
Please refer to lot specific datasheet.
Host
E. coli
Specificity
Two or more units of ADP-ribose
Species Reactivity
Mouse
Human
Species Reactivity Note
Mouse, Human. Predicted to react with all species.
Purification Method
Ni-NTA agarose
Molecular Weight
Variable depending on the target proteins and the extend of ADP-ribosylation
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting on ADP-ribosylated PARP1 and PARP3 recombinant proteins.
Western Blotting Analysis: This reagent detected oligo(ADPR) and poly(ADPR) on ADP-ribosylated PARP1 recombinant protein (Lee Kraus, University of Texas Southwestern Medical Center).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
