| Description | |
|---|---|
| Catalogue Number | MABE1142 |
| Description | Anti-ZP-2 Antibody, clone IE-3 |
| Alternate Names |
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| Background Information | Zona pellucida sperm-binding protein 2 (UniProt: P20239; also known as Zona pellucida glycoprotein 2, Zp-2, Zona pellucida protein A) is encoded by the Zp2 (also known as Zp-2, Zpa) gene (Gene ID: 22787) in murine species. The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The mammalian zona pellucida mediates species-specific sperm binding, induction of the acrosome reaction, and prevents post-fertilization polyspermy. ZP2 is expressed during the 2-week growth phase of oogenesis, prior to ovulation and may act as a secondary sperm receptor and is proteolytically cleaved in the N-terminal part by the metalloendopeptidase ASTL and is exocytosed from cortical granules after fertilization, yielding an N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage plays an important role in the post-fertilization block to polyspermy. Female mice lacking Zp2 gene do not form a stable zona matrix and are sterile. (Ref.: Hinsch, KD and Hinsch, E. (1999). Andrologia.31(5):320-322.) |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mm NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-ZP-2, clone IE-3, Cat. No. MABE1142, is a highly specific rat monoclonal antibody that targets Zona pellucida sperm-binding protein 2 and has been tested in ELISA, Immunofluorescence, Immunoprecipitation, and Western Blotting. |
| Key Applications |
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| Application Notes | Immunoprecipitation Analysis: A representative lot detected ZP-2 in Immunoprecipitation applications (East, I.J., et. Al. (1984). J Cell Biol. 98(3):795-800). Immunofluorescence Analysis: A representative lot detected ZP-2 in Immunofluorescence applications (East, I.J., et. Al. (1984). J Cell Biol. 98(3):795-800). Immunofluorescence A representative lot detected ZP-2 in Immunofluorescence applications (Avella, M.A., et. Al. (2016). Sci Transl Med. 8(336):336ra60). ELISA Analysis: A representative lot detected ZP-2 in Western Blotting applications (East, I.J., et. Al. (1984). J Cell Biol. 98(3):795-800; Sun, W., et. Al. (1999). Biol Reprod. 60(4):900-7). Western Blotting Analysis: A representative lot detected ZP-2 in ELISA applications (Sun, W., et. Al. (1999). Biol Reprod. 60(4):900-7). |
| Biological Information | |
|---|---|
| Immunogen | Purified mouse zonae pellucidae. |
| Epitope | N-terminus |
| Clone | IE-3 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rat |
| Specificity | Clone IE-3 detects Zona pellucida sperm-binding protein 2 in murine species. |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Species Reactivity Note | Mouse. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein g purified |
| UniProt Number |
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| Molecular Weight | ~120 kDa observed; 80.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse ovary tissue lysate. Western Blotting Analysis: 1 µg/mL of this antibody detected ZP-2 in 10 µg of mouse ovary tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
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| Material Size | 100 μg |