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Millipore This Anti-Jarid1d Antibody, Clone 7f10.4 Is Validated For Use In Western Blotting And Immunocytochemistry For The Detection Of Kdm5d . - Mill (Additional S&H or Hazmat Fees May Apply)
Lysine-specific demethylase 5D (UniProt Q9BY66; also known as H-Y, Histone demethylase JARID1D, Histocompatibility Y antigen, Human homolog of selected mouse cDNA on Y, Jumonji/ARID domain-containing protein 1D, Jumonji AT rich interactive domain 1D (RBP2-like), Protein SmcY, SMC homolog Y chromosome, Smcy homolog Y-linked) is encoded by the KDM5D (also known as HY, HYA, JARID1D, KIAA0234, SMCY) gene (Gene ID 8284) in human. JARID1D/KDM5D is a histone demethylase that specifically catalyzes the demethylation of tri- and di-methylated Lys4 of histone H3 (H3K4m3 and H3K4m2), but not methylated H3K9, H3K27, H3K36, H3K79, H4K20, or monomethylated H3K4. The KDM5D gene is mapped to the azoospermia factor (AZF) region known to be involved in spermatogenesis.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-JARID1D Antibody, clone 7F10.4 is validated for use in Western Blotting and Immunocytochemistry for the detection of KDM5D .
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected JARID1D/KDM5D in HeLa cells.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to human JARID1D/KDM5D.
Clone
7F10.4
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 7F10.4 is expected to react with all three spliced JARID1D/KDM5D isoforms.
Isotype
IgG1κ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001140177
Gene Symbol
KDM5D
HY
HYA
JARID1D
KIAA0234
SMCY
Purification Method
Protein G Purified
UniProt Number
Q9BY66
Molecular Weight
~200 kDa observed. Isoform 1 (174.1 kDa calculated); Isoform 2 (167.4 kDa calculated); Isoform 3 (177.6 kDa calculated). Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in U2OS cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected JARID1D/KDM5D in 10 µg of U2OS cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
