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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE344 |
| Description | Anti-BICC1/Protein Bicaudal C Homolog 1 Antibody, clone 2D9.1 |
| Alternate Names |
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| Background Information | Protein bicaudal C homolog 1 (UniProt Q9H694; also known as Bic-C) is encoded by the BICC1 (also known as CYSRD) gene (Gene ID 80114) in human. Drosophila bicaudal C and its mammalian homologs are RNA-binding proteins involved in symmetric distribution and localized translation of mRNAs of many proteins in a wide range of cells and tissues. RNA binding of Bic-C is mediated by N-terminal KH domains (a.a. 132-199 & 284-348), whereas a C-terminal sterile alpha motif (SAM; a.a. 873-936) self-polymerizes and localizes Bic-C at cytoplasmic foci where silencing of bound mRNAs takes place. Defective polymerization results in decreased Bic-C stability and thereby attenuates inhibition of Dishevelled-2 of Wnt/β-catenin pathway. Loss of BICC1 provokes renal and pancreatic cysts as well as ectopic Wnt/β-catenin signaling during visceral left-right patterning due to defective silencing of Bic-C target mRNAs, including adenylate cyclase 6 (AC6). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-BICC1 Antibody, clone 2D9.1 is an antibody against Protein Bicaudal C Homolog 1 for use in Western Blotting, Immunoprecipitation. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1 µg/mL from a representative lot detected Bic-C/BICC1 in human HK and murine mIMCD-3 kidney cell lysates (Courtesy of Dr. Thomas Tuschl laboratory, The Rockefeller University, New York, NY) Immunoprecipitation Analysis: 9 µg from a representative lot immunoprecipitated exogenously expressed human BICC1 isoforms 1 and 2 from 0.5 mL (~50-100 mg) of HEK293 transfectants lysates (Courtesy of Dr. Thomas Tuschl laboratory, The Rockefeller University, New York, NY). |
| Biological Information | |
|---|---|
| Immunogen | Recombinant fragment corresponding to the N-terminal half of human BICC1/Protein Bicaudal C Homolog 1. |
| Epitope | N-terminal half. |
| Clone | 2D9.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Clone 2D9.1 reacts with both spliced isoforms of human and murine BICC1 reported by UniProt (Q9H694 & Q99MQ1). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~130 kDa observed. 104.8/105.0 kDa (Human/mouse isoform 1) and 94.29/93.41 kDa (Human/mouse isoform 2) calculated. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting of recombinant human BICC1. Western Blotting Analysis: 0.2 µg/mL of this antibody detected 10 µg of recombinant human BICC1. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |