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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE354 |
| Description | Anti-Uracil-DNA glycosylase Antibody, clone 8G10.1 |
| Alternate Names |
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| Background Information | Uracil-DNA glycosylase (EC 3.2.2.27; UniProt P13051; also known as UDG) is encoded by the UNG (also known as DGU, HIGM4, HIGM5, UNG1, UNG2, UNG15) gene (Gene ID 7374) in human. Uracil-DNA glycosylases catalyze the release of uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway that plays an important role in mutagenesis prevention. Uracil bases in DNA can result from cytosine deamination or misincorporation of dUMP instead of dTTP during DNA synthesis. Alternative promoter usage and splicing of UNG gene lead to two different isoforms, the mitochondrial UNG1 and the nuclear UNG2. The mammalian shelterin complex contains the six proteins (TRF1, TRF2, RAP1, TIN2, POT1, and TPP1), the complex occupies the end of the telomeres and protect telomeres against inappropriate DNA repair when telomeres are not being lengthened. UNG deficiency in primary mouse hematopoietic cells is shown to affect POT1 (Protector of Telomeres 1) recognition of the telomere repeats due to increased uracil incorporation in telomeres TTAGGG repeats, which in turn leads to abnormal telomere lengthening. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Uracil-DNA glycosylase Antibody, clone 8G10.1, Cat. No. MABE354, is a highly specific mouse monoclonal antibody, that targets UNG and has been tested in Western Blotting and Immunohistochemistry. |
| Key Applications |
|
| Application Notes | Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Uracil-DNA glycosylase in human skeletal muscle and human cardiac tissues. |
| Biological Information | |
|---|---|
| Immunogen | Linear peptide corresponding to human Uracil-DNA glycosylase near the C-terminus. |
| Epitope | Near C-terminus |
| Clone | 8G10.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with both spliced isoforms (UNG1 & UNG2) of human Uracil-DNA glycosylase. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Species Reactivity Note | Human. Predicted to react with Monkey, Chimpanzee based on 100% sequence homology. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~34 kDa observed (33.9 kDa/UNG1 & 34.6 kDa/UNG2 calculated). Immunogen sequence should allow to detect both isoform 1 and 2. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in HepG2 cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Uracil-DNA glycosylase in 10 µg of HepG2 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |