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Millipore This Anti-Bptf Antibody, Clone 15b1.1 Is Validated For Use In Western Blotting And Immunohistochemistry (Paraffin) For The Detection Of Bptf . - Mill (Additional S&H or Hazmat Fees May Apply)
Nucleosome-remodeling factor (NURF) subunit BPTF (UniProt E9Q6A7 & A2A654; also known as Bromodomain and PHD finger-containing transcription factor, Fetal Alzheimer antigen) is encoded by the Bptf (also known as 9430093H17Rik, Falz) gene (Gene ID 207165) in murine species. ATP-dependent chromatin remodeling complexes are classified into four major subfamilies (SWI/SNF, ISWI, CHD, and INO80). NURF is the founding member of ISWI remodeling complexes. Mammalian NURF is composed of BPTF, the ATPase SNF2L, and the Trp-Asp (WD) repeat protein pRBAP46/48, with BPTF being the largest subunit among the three. BPTF PHD finger-mediated binding of H3K4me3 stabilizes the NURF complex on chromatin, resulting in NURF-catalyzed nucleosome sliding. The NURF remodeling complex is essential for mammalian development and executes epigenetic regulation in multiple signaling pathways, including heat shock, TGF-beta/SMAD, JAK/STAT, WNT/beta-catenin, RB, and nuclear hormone receptors.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-BPTF Antibody, clone 15B1.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of BPTF .
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected BPTF in human testis, rat colon, and mouse colon tissue.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to mouse BPTF.
Clone
15B1.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG2bκ
Species Reactivity
Human
Mouse
Rat
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_789820
Gene Symbol
Bptf
Purification Method
Protein G Purified
UniProt Number
E9Q6A7
Molecular Weight
~322 kDa observed. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected BPTF in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
