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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, R | WB, IHC, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE461 |
| Description | Anti-CRTC1 Antibody/TORC-1, clone 5F3.1 |
| Alternate Names |
|
| Background Information | CREB-regulated transcription coactivator 1 (UniProt Q6UUV9; also known as Mucoepidermoid carcinoma translocated protein 1, TORC-1, Transducer of CREB protein 1, Transducer of regulated cAMP response element-binding protein 1) is encoded by the CRTC1 (also known as KIAA0616, MECT1, TORC1, WAMTP1) gene (Gene ID 23373) in human. CRTC1 belongs to a family of glycosylated Ser/Thr-rich phosphoproteins that bind the basic leucine zipper (bZIP) region of Ca2+/cAMP-responsive element-binding protein (CREB) and function as CREB coactivators. CRTCs participate in CREB-mediated transcriptions of both canonical cellular cAMP response elements (CREs) and the non-canonical viral CREs. CRTC1, specifically, is involved in CREB-mediated transcription of various neuronal immediate early genes (IEGs) as well as CREB-dependent hepatitis B virus (HBV) replication. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-CRTC1 Antibody/TORC-1, clone 5F3.1 is validated for use in Western Blotting, Immunohistochemistry, Immunoprecipitation for the detection of CRTC1. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 1.0 µg/mL from a representative lot detected CRTC1/TORC-1 in 10 µg of mouse hypothalamus tissue lysate. Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected CRTC1/TORC-1 in human cerebral cortex, rat cerebral cortex, human cerebellum, and rat cerebellum tissue. Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous CRTC1/TORC-1 from mouse hippocampus tissue lysates, as well as exogenously expressed murine CRTC1/TORC-1, but not CRTC2/TORC-2, from lysates of transfected HEK293 cells (Courtesy of Dr. Carlos A. Saura , Universitat Autònoma de Barcelona, Spain). Western Blotting Analysis: A representative lot detected endogenous CRTC1/TORC-1 in human, mouse, and rat hippocampus tissue lysates, as well as exogenously expressed murine CRTC1/TORC-1 in lysates from transfected HEK293 cells (Courtesy of Dr. Carlos A. Saura , Universitat Autònoma de Barcelona, Spain). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human CRTC1/TORC-1 sequence within the N-terminal half.. |
| Epitope | Internal (N-terminal half) |
| Clone | 5F3.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with all three human spliced isoforms |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein G Purified |
| UniProt Number |
|
| Molecular Weight | ~68 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse cerebellum tissue lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected CRTC1/TORC-1 in 10 µg of mouse cerebellum tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |