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Millipore This Anti-Monomethyl Histone H3 (Lys4) Antibody, Clone Rm140 Is Validated For Use In Western Blotting, Multiplexing For The Detection Of Monomethyl Histone H3. - Mill (Additional S&H or Hazmat Fees May Apply)
Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the beads on a string structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.
Product Information
Format
Purified
Presentation
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-monomethyl Histone H3 (Lys4) Antibody, clone RM140 is validated for use in Western Blotting, Multiplexing for the detection of monomethyl Histone H3.
Key Applications
Western Blotting
Multiplexing
Application Notes
Multiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.
Biological Information
Immunogen
A linear peptide corresponding to human Histone H3 monomethylated at Lys4.
Clone
RM140
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This antibody reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2) or trimethylated Lysine 4 (K4me3), or other methylations in Histone H3.
Isotype
IgG
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
H3F3A
H3.3A
H3F3
PP781
H3F3B
H3.3B
Purification Method
Protein A Purfied
UniProt Number
P84243
Molecular Weight
~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Recombinant HeLa protein and HeLa acid extract.
Western Blotting Analysis (WB): 0.5 μg/mL of this antibody detected monomethyl Histone H3 (Lys4) in HeLa acid extract.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
