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Millipore This Anti-Monomethyl Histone H3 (Lys79), Clone Rm147 Antibody Is Validated For Use In Western Blotting, Multiplexing For The Detection Of Monomethyl Histone H3. - Mill (Additional S&H or Hazmat Fees May Apply)
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Lysine methylation occurs in three distinct states, having either one (me1), two (me2) or three (me3) methyl groups attached to the amine group of the lysine side chain. Methylation of Lysine 79 in Histone 3 acts as a marker of inactive chromatin regions. Methylation by Dot1 methylase on lysine-79 (Lys79) of Histone H3 is criticial for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation.
Product Information
Format
Purified
Presentation
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-monomethyl Histone H3 (Lys79), clone RM147 Antibody is validated for use in Western Blotting, Multiplexing for the detection of monomethyl Histone H3.
Key Applications
Western Blotting
Multiplexing
Application Notes
Multiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.
Biological Information
Immunogen
A linear peptide corresponding to human Histone H3 monomethylated at Lys79.
Clone
RM147
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This antibody reacts to Histone H3 monomethylated at Ly79 (K79me1). No cross reactivity with dimethylated Lys79 (K79me2) or trimethylated Lys79 (K79me3), or other methylations in histone H3.
Isotype
IgG
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Gene Symbol
H3F3A
H3.3A
H3F3
PP781
H3F3B
H3.3B
Purification Method
Protein A Purfied
UniProt Number
P84243
Molecular Weight
~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa acid extract and HeLa cell lysate.
Western Blotting Analysis (WB): 0.5 μg/mL of this antibody detected monomethyl Histone H3 (Lys79) in HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
