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Millipore Anti-Heterochromatin Protein-1 , Clone 4c12.1, Cat. No. Mabe666, Is A Highly Specific Mouse Monoclonal Antibody That Targets Heterochromatin Protein-1 Alpha And Has Been - Mill (Additional S&H or Hazmat Fees May Apply)
Chromobox protein homolog 5 (UniProt: P45973; also known as Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha) is encoded by the CBX5 (also known as HP1A) gene (Gene ID: 23468) in human. Chromobox protein homolog 5 is a component of centromeric and pericentromeric heterochromatin that associates with chromosomes during mitosis. Chromobox protein homolog 5 associates specifically with chromatin during metaphase and anaphase. It recognizes and binds histone H3 tails methylated at Lys9 (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when Tyr41 of histone H3 is phosphorylated. It can interact with lamin-B receptor (LBR) and this interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. It is also involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-Heterochromatin Protein-1 , clone 4C12.1, Cat. No. MABE666, is a highly specific mouse monoclonal antibody that targets Heterochromatin Protein-1 alpha and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
Immunohistochemistry (Paraffin)
Western Blotting
Application Notes
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Heterochromatin Protein-1 in human tonsil, human pancreas, and human uterus tissues.
Biological Information
Immunogen
GST/His-tagged recombinant fraction corresponding to 68 amino acids from the internal region of human Heterochromatin protein 1 homolog alpha.
Clone
4C12.1
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG2aκ
Species Reactivity
Human
Mouse
Species Reactivity Note
Human, Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001120793
Gene Symbol
CBX5
HP1A
Purification Method
Protein G purified
UniProt Number
P45973
Molecular Weight
~23 kDa observed; 22.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in NIH/3T3 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Heterochromatin Protein-1 in 10 µg of NIH/3T3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
