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Millipore Anti-Atrx Antibody, Clone 23c Is An Antibody Against Atrx For Use In Western Blotting And Immunocytochemistry. - Mill (Additional S&H or Hazmat Fees May Apply)
Transcriptional regulator ATRX (EC 3.6.4.12; UniProt P46100; also known as Alpha thalassemia/mental retardation syndrome X-linked, ATP-dependent helicase ATRX, DNA dependent ATPase and helicase, Helicase 2 X-linked, X-linked helicase II, X-linked nuclear protein, XNP, Zinc finger helicase, Znf-HX) is encoded by the PRF1 (Also known as ATR2, JMS, MRXHF1, RAD54, RAD54L, SFM1, SHS, XH2, XNP, ZNF-HX) gene in human (Gene ID 546). Rad54 belongs to the SWI/SNF family of chromatin remodeling proteins. Rad54 undergoes cell cycle-dependent phosphorylation, nuclear matrix and chromatin association, and is implicatetd in playing a role in gene regulation during interphase and mitosis. ATRX mutations are associated with an X-linked mental retardation (XLMR) syndrome. most often accompanied by alpha-thalassemia syndrome, as a result of diverse changes in DNA methylation.
Product Information
Format
Purified
Presentation
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-ATRX Antibody, clone 23C is an antibody against ATRX for use in Western Blotting and Immunocytochemistry.
Key Applications
Western Blotting
Immunocytochemistry
Application Notes
Western Blotting Analysis: A representative lot detected ATRX in nuclear extracts from EBV-transformed lymphocytes (McDowell, T.L., et al. (1999). PNAS. 96(24):13983-13988). Immunocytochemistry Analysis: A representative lot detected nuclear localization of ATRX in HeLa and L929 cells (McDowell, T.L., et al. (1999). PNAS. 96(24):13983-13988).
Biological Information
Immunogen
Recombinant protein corresponding to the N-terminus of human ATRX.
Epitope
N-terminus
Clone
23C
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Isotype
IgG1κ
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000480
Gene Symbol
ATRX
RAD54L
XH2
Purification Method
Protein G Purified
UniProt Number
P46100
Molecular Weight
~283 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected ATRX in 10 µg of HeLa cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
