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Millipore This Anti-Pax-2 Antibody, Clone 4c2.2 Is Validated For Use In Western Blotting For The Detection Of Pax-2 . - Mill (Additional S&H or Hazmat Fees May Apply)
Paired box protein Pax-2 (UniProt Q02962; also known as Paired box homeotic gene 2) is encoded by the PAX2 (also known as FSGS7, PAPRS) gene (Gene ID 5076) in human. Pax-2 belongs to the family of transcription factors with conserved DNA-binding paired box domain. The family of paired box (PAX) genes is divided into four subgroups (I–IV). Genes in subgroups II (PAX2, PAX5, and PAX8) and III (PAX3 and PAX7) have been implicated in tumor development. PAX2 is a critical regulator of embryogenesis and it is normally expressed in the pancreas and in subpopulations of nodal lymphocytes in adult tissues. Aberrant PAX2 expression is frequently observed in several cancer types.
Product Information
Format
Culture Supernatant
Presentation
Mouse monoclonal IgG2bκ antibody in buffer containing supernatant without preservatives.
Quality Level
MQ100
Applications
Application
This Anti-Pax-2 Antibody, clone 4C2.2 is validated for use in Western Blotting for the detection of Pax-2 .
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A 1:500 dilution from a representative lot detected PAX-2 in 10 µg of HeLa and PC3 cell lysate.
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human Pax-2.
Clone
4C2.2
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone 4C2.2 is expected to react with spliced Pax-2 isoform 1 and isoform 2 (Fetal kidney isoform). Reactivity toward isoforms 3&4 has not been tested.
Isotype
IgG2bκ
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_000269
Gene Symbol
PAX2
Purification Method
Unpurified
UniProt Number
Q02962
Molecular Weight
~45 kDa observed. ~45 kDa observed. Isoform 1 (44.7 kDa calculated); Isoform 2 (44.5 kDa calculated). An uncharacterized band ~17 kDa is seen in some lysate samples.
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected PAX-2 in 10 µg of A431 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
