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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE972 |
| Description | Anti-PRDM5 Antibody |
| Alternate Names |
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| Background Information | PR domain zinc finger protein 5 (UniProt Q9NQX1) is encoded by the PRDM1 gene (also known as BCS2, PFM2) in human (Gene ID 11107). PRDM proteins constitute a family of transcriptional regulators that are characterized by the presence of variable numbers of zinc finger domains and an N-terminal PR domain. PRDM5 can negatively regulate gene transcriptions by recruiting the G9a histone methyltransferase and histone deacetylases. PRDM5 can promote transcription by targeting enhancer-like elements or recruiting RNA polymerase II binding. PRDM5 is highly expressed in murine embryonic stem cells (mESCs) and associates with complexes involved in chromatin organization, such as Ctcf, Smc1 (cohesin), and TFIIIC, which indicates their involvement in chromatin organization. PRDM5 is often found silenced in human breast, ovarian, liver, and cervical cancers. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-PRDM5 Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of PRDM5 . |
| Key Applications |
|
| Application Notes | Immunoprecipitation Analysis: A representative lot immunoprecipitated PRDM5 in mouse embryonic fibroblasts (Data courtesy of Dr. Anders H. Lund, University of Copenhagen, Denmark). Immunoprecipitation: A representative lot of this antibody immunoprecipitated PRDMS E9 and E10 intrahepatic cholangiocarcinoma (ICC) cells. (Seehawer, M., et al. (2018). Nature 562(7725); 69-75). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to human PRDM5 near the N-terminus. |
| Epitope | Near N-terminus |
| Clone | 57-20 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with all four spliced isoforms |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~73 kDa observed. 73.1 kDa (isoform 1, calculated), 69.9 kDa (isoform 2, calculated), 12.9 kDa (isoform 3, calculated), 58.1 kDa (isoform 4, calculated). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in mouse ESC lysate. Western Blotting Analysis: 8.0 µg/mL of this antibody detected PRDM5 in 10 µg of mouse ESC lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 200 μg |