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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | WB, IHC, ICC, RIP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE985 |
| Description | Anti-Fox1 Antibody, clone 1D10 |
| Alternate Names |
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| Background Information | RNA binding protein fox-1 homolog 1 (UniProt Q9JJ43; also known as Ataxin-2-binding protein 1, Fox-1 homolog A, Hexaribonucleotide binding protein 1) is encoded by the Rbfox1 (also known as A2bp, A2bp1, Fox-1, Hrnbp1) gene (ORF MNCb-3035; Gene ID 268859) in murine species. The RNA-binding Fox (Rbfox) family of splicing factors is comprised of three members, Rbfox1 (Fox-1 or A2BP1), Rbfox2 (Fox-2 or RBM9), and Rbfox3 (Fox-3, HRNBP3 or NeuN). Rbfox proteins regulate splicing of many neuronal transcripts (pre-mRNAs) by binding the sequence (U)GCAUG in introns flanking alternative exons. A (U)GCAUG motif that lies downstream of the alternative exon generally promotes Rbfox-dependent exon inclusion, whereas an upstream motif will usually repress exon inclusion. Rbfox1 transcript itself is subjected to alternative splicing in response to chronic cell depolarization. This results in the increased expression of the nuclear, splicing-active Rbfox1 isoform, which in turn leads to increased splicing of Rbfox1 target transcripts of proteins, including N-methyl D-aspartate (NMDA) receptor 1 (Grin1) and a calcium ATPase (Atp2b1), that play important roles in neuronal excitation and calcium homeostasis. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Fox1 Antibody, clone 1D10, Cat. No. MABE985, is a highly specific mouse Monoclonal antibody, that targets RNA binding protein fox-1 homolog 1 and has been tested in Western Blotting, Immunocytochemistry, Immunohistochemistry and RNA-Binding Immunoprecipitation/iCLIP. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Fox1 in 10 µg of adult mouse frontal cortex tissue lysate. Immunohistochemistry Analysis: A representative lot detected Fox1 in coronal sections from E18 mouse (Tang, Z.Z., et al. (2009). Mol Cell Biol. 29(17):4757-4765). Immunocytochemistry Analysis: A representative lot detected Fox1 in post-mitotic neuronal cells differentiated from mouse embryonal carcinoma P19 cells (Lee, J.A., et al. (2009). Genes Dev. 23(19):2284-2293). RNA-Binding Protein Immunoprecipitation/iCLIP Analysis: A representative lot was employed to immunoprecipitate Fox1-associated pre-mRNAs from UV-crosslinked murine cortex homogenates by individual-nucleotide resolution crosslinking immunoprecipitation (iCLIP) for characterization of Fox1 pre-mRNA-binding sites (Gehman, L.T., et al. (2011) Nat Genet. 43(7):706-711). Western Blotting Analysis: A representative lot detected Fox1 in mouse cortical tissue homogenate (Tang, Z.Z., et al. (2009). Mol Cell Biol. 29(17):4757-4765). |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant protein corresponding to mouse Fox1. |
| Clone | 1D10 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Isotype | IgG2aκ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~43 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in adult mouse brain tissue lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fox1 in 10 µg of adult mouse brain tissue lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |