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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABE990 |
| Description | Anti-MSL-2 Antibody, clone 10C3.1 |
| Alternate Names |
|
| Background Information | E3 ubiquitin-protein ligase MSL2 (UniProt Q9HCI7; also known as Male-specific lethal-2 homolog, Male-specific lethal-2 homolog 1, Male-specific lethal 2-like 1, MSL-2, MSL2-like 1, RING finger protein 184) is encoded by the MSL2 (also known as KIAA1585, MSL2L1, RNF184) gene (Gene ID 55167) in human. MSL2 (male-specific lethal 2) was originally identified in Drosophila and characterized as a gene whose mution causes male-specific lethality. Human MSL (hMSL) orthologues are found in an evolutionary conserved complex known as the hMOF (Males absent on the first, human) complex that is composed of hMOF, hMSL1, hMSL2, hMSL3, and Nucleoprotein 153 (NUP153). MSL2 is a RING domain-containing E3 ligase that targets the tumor suppressor p53 Lys351 for monoubiquitination, exposing a nuclear export motif within p53 as well as releasing p53 from MDM2. MSL2 overexpression causes an upregulation of mitochondrial-dependent apoptosis as a result of p53 cytoplasmic accumulation. hMSL1/hMSL2 heterodimer is reported to ubiquitinate histone H2B on Lys34 (H2BK34ub), which promotes transcription activation by stimulating the activities of MLL/SET1 family H3K4 methyltransferases and the H3K79 methyltransferase DOT1L. In addition, both hMSL1 and hMOF are reported to be modified by hMSL2 following DNA damage induction, and hMSL2-depletion causes impaired non-homologous end joining (NHEJ) repair in human cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-MSL-2 Antibody, clone 10C3.1 is an antibody against MSL-2 for use in Western Blotting, Immunocytochemistry. |
| Key Applications |
|
| Application Notes | Western Blotting Analysis: 2.0 µg/mL of this antibody detected MSL-2 in 10 µg of HepG2 and HEK293 cell lysates. Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected MSL-2 in HUVECs, A431 and HeLa cells. |
| Biological Information | |
|---|---|
| Immunogen | GST-tagged recombinant human MSL-2 C-terminal fragment. |
| Epitope | Internal (C-terminal half). |
| Clone | 10C3.1 |
| Concentration | Please refer to lot specific datasheet. |
| Host | Mouse |
| Specificity | Expected to react with both spliced isoforms of human MSL2 reported by UniProt (Q9HCI7). |
| Isotype | IgG1κ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~62 kDa observed. 62.54 kDa (isoform 1) and 54.34 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in LNCaP cell lysate. Western Blotting Analysis: 2.0 µg/mL of this antibody detected MSL-2 in 10 µg of LNCaP cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |